Protein kinase C epsilon activity in the nucleus accumbens and central nucleus of the amygdala mediates binge alcohol consumption

Debra K. Cozzoli, Justin Courson, Charlotte Rostock, Rianne R. Campbell, Melissa G. Wroten, Hadley McGregor, Amanda L. Caruana, Bailey W. Miller, Jia Hua Hu, Pingwu Zhang, Bo Xiao, Paul F Worley, John C. Crabbe, Deborah A. Finn, Karen K. Szumlinski

Research output: Contribution to journalArticle

Abstract

background Protein kinase C epsilon (PKCε) is emerging as a potential target for the development of pharmacotherapies to treat alcohol use disorders, yet little is known regarding how a history of a highly prevalent form of drinking, binge alcohol intake, influences enzyme priming or the functional relevance of kinase activity for excessive alcohol intake. Methods Immunoblotting was employed on tissue from subregions of the nucleus accumbens (NAc) and the amygdala to examine both idiopathic and binge drinking-induced changes in constitutive PKCε priming. The functional relevance of PKCε translocation for binge drinking and determination of potential upstream signaling pathways involved were investigated using neuropharmacologic approaches within the context of two distinct binge drinking procedures, drinking in the dark and scheduled high alcohol consumption. Results Binge alcohol drinking elevated p(Ser729)-PKCε levels in both the NAc and the central nucleus of the amygdala (CeA). Moreover, immunoblotting studies of selectively bred and transgenic mouse lines revealed a positive correlation between the propensity to binge drink alcohol and constitutive p(Ser729)-PKCε levels in the NAc and CeA. Finally, neuropharmacologic inhibition of PKCε translocation within both regions reduced binge alcohol consumption in a manner requiring intact group 1 metabotropic glutamate receptors, Homer2, phospholipase C, and/or phosphotidylinositide-3 kinase function. Conclusions Taken together, these data indicate that PKCε signaling in both the NAc and CeA is a major contributor to binge alcohol drinking and to the genetic propensity to consume excessive amounts of alcohol.

Original languageEnglish (US)
Pages (from-to)443-451
Number of pages9
JournalBiological Psychiatry
Volume79
Issue number6
DOIs
StatePublished - Mar 15 2016

Fingerprint

Protein Kinase C-epsilon
Binge Drinking
Nucleus Accumbens
Alcohol Drinking
Alcohols
Immunoblotting
Phosphotransferases
Type C Phospholipases
Central Amygdaloid Nucleus
Amygdala
Transgenic Mice
Drinking
Drug Therapy
Enzymes

Keywords

  • Alcohol use disorders
  • Drinking in the dark
  • Glutamate
  • HDID-1 mice
  • Homer
  • Scheduled high alcohol consumption

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Cozzoli, D. K., Courson, J., Rostock, C., Campbell, R. R., Wroten, M. G., McGregor, H., ... Szumlinski, K. K. (2016). Protein kinase C epsilon activity in the nucleus accumbens and central nucleus of the amygdala mediates binge alcohol consumption. Biological Psychiatry, 79(6), 443-451. https://doi.org/10.1016/j.biopsych.2015.01.019

Protein kinase C epsilon activity in the nucleus accumbens and central nucleus of the amygdala mediates binge alcohol consumption. / Cozzoli, Debra K.; Courson, Justin; Rostock, Charlotte; Campbell, Rianne R.; Wroten, Melissa G.; McGregor, Hadley; Caruana, Amanda L.; Miller, Bailey W.; Hu, Jia Hua; Zhang, Pingwu; Xiao, Bo; Worley, Paul F; Crabbe, John C.; Finn, Deborah A.; Szumlinski, Karen K.

In: Biological Psychiatry, Vol. 79, No. 6, 15.03.2016, p. 443-451.

Research output: Contribution to journalArticle

Cozzoli, DK, Courson, J, Rostock, C, Campbell, RR, Wroten, MG, McGregor, H, Caruana, AL, Miller, BW, Hu, JH, Zhang, P, Xiao, B, Worley, PF, Crabbe, JC, Finn, DA & Szumlinski, KK 2016, 'Protein kinase C epsilon activity in the nucleus accumbens and central nucleus of the amygdala mediates binge alcohol consumption', Biological Psychiatry, vol. 79, no. 6, pp. 443-451. https://doi.org/10.1016/j.biopsych.2015.01.019
Cozzoli, Debra K. ; Courson, Justin ; Rostock, Charlotte ; Campbell, Rianne R. ; Wroten, Melissa G. ; McGregor, Hadley ; Caruana, Amanda L. ; Miller, Bailey W. ; Hu, Jia Hua ; Zhang, Pingwu ; Xiao, Bo ; Worley, Paul F ; Crabbe, John C. ; Finn, Deborah A. ; Szumlinski, Karen K. / Protein kinase C epsilon activity in the nucleus accumbens and central nucleus of the amygdala mediates binge alcohol consumption. In: Biological Psychiatry. 2016 ; Vol. 79, No. 6. pp. 443-451.
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abstract = "background Protein kinase C epsilon (PKCε) is emerging as a potential target for the development of pharmacotherapies to treat alcohol use disorders, yet little is known regarding how a history of a highly prevalent form of drinking, binge alcohol intake, influences enzyme priming or the functional relevance of kinase activity for excessive alcohol intake. Methods Immunoblotting was employed on tissue from subregions of the nucleus accumbens (NAc) and the amygdala to examine both idiopathic and binge drinking-induced changes in constitutive PKCε priming. The functional relevance of PKCε translocation for binge drinking and determination of potential upstream signaling pathways involved were investigated using neuropharmacologic approaches within the context of two distinct binge drinking procedures, drinking in the dark and scheduled high alcohol consumption. Results Binge alcohol drinking elevated p(Ser729)-PKCε levels in both the NAc and the central nucleus of the amygdala (CeA). Moreover, immunoblotting studies of selectively bred and transgenic mouse lines revealed a positive correlation between the propensity to binge drink alcohol and constitutive p(Ser729)-PKCε levels in the NAc and CeA. Finally, neuropharmacologic inhibition of PKCε translocation within both regions reduced binge alcohol consumption in a manner requiring intact group 1 metabotropic glutamate receptors, Homer2, phospholipase C, and/or phosphotidylinositide-3 kinase function. Conclusions Taken together, these data indicate that PKCε signaling in both the NAc and CeA is a major contributor to binge alcohol drinking and to the genetic propensity to consume excessive amounts of alcohol.",
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AU - Cozzoli, Debra K.

AU - Courson, Justin

AU - Rostock, Charlotte

AU - Campbell, Rianne R.

AU - Wroten, Melissa G.

AU - McGregor, Hadley

AU - Caruana, Amanda L.

AU - Miller, Bailey W.

AU - Hu, Jia Hua

AU - Zhang, Pingwu

AU - Xiao, Bo

AU - Worley, Paul F

AU - Crabbe, John C.

AU - Finn, Deborah A.

AU - Szumlinski, Karen K.

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N2 - background Protein kinase C epsilon (PKCε) is emerging as a potential target for the development of pharmacotherapies to treat alcohol use disorders, yet little is known regarding how a history of a highly prevalent form of drinking, binge alcohol intake, influences enzyme priming or the functional relevance of kinase activity for excessive alcohol intake. Methods Immunoblotting was employed on tissue from subregions of the nucleus accumbens (NAc) and the amygdala to examine both idiopathic and binge drinking-induced changes in constitutive PKCε priming. The functional relevance of PKCε translocation for binge drinking and determination of potential upstream signaling pathways involved were investigated using neuropharmacologic approaches within the context of two distinct binge drinking procedures, drinking in the dark and scheduled high alcohol consumption. Results Binge alcohol drinking elevated p(Ser729)-PKCε levels in both the NAc and the central nucleus of the amygdala (CeA). Moreover, immunoblotting studies of selectively bred and transgenic mouse lines revealed a positive correlation between the propensity to binge drink alcohol and constitutive p(Ser729)-PKCε levels in the NAc and CeA. Finally, neuropharmacologic inhibition of PKCε translocation within both regions reduced binge alcohol consumption in a manner requiring intact group 1 metabotropic glutamate receptors, Homer2, phospholipase C, and/or phosphotidylinositide-3 kinase function. Conclusions Taken together, these data indicate that PKCε signaling in both the NAc and CeA is a major contributor to binge alcohol drinking and to the genetic propensity to consume excessive amounts of alcohol.

AB - background Protein kinase C epsilon (PKCε) is emerging as a potential target for the development of pharmacotherapies to treat alcohol use disorders, yet little is known regarding how a history of a highly prevalent form of drinking, binge alcohol intake, influences enzyme priming or the functional relevance of kinase activity for excessive alcohol intake. Methods Immunoblotting was employed on tissue from subregions of the nucleus accumbens (NAc) and the amygdala to examine both idiopathic and binge drinking-induced changes in constitutive PKCε priming. The functional relevance of PKCε translocation for binge drinking and determination of potential upstream signaling pathways involved were investigated using neuropharmacologic approaches within the context of two distinct binge drinking procedures, drinking in the dark and scheduled high alcohol consumption. Results Binge alcohol drinking elevated p(Ser729)-PKCε levels in both the NAc and the central nucleus of the amygdala (CeA). Moreover, immunoblotting studies of selectively bred and transgenic mouse lines revealed a positive correlation between the propensity to binge drink alcohol and constitutive p(Ser729)-PKCε levels in the NAc and CeA. Finally, neuropharmacologic inhibition of PKCε translocation within both regions reduced binge alcohol consumption in a manner requiring intact group 1 metabotropic glutamate receptors, Homer2, phospholipase C, and/or phosphotidylinositide-3 kinase function. Conclusions Taken together, these data indicate that PKCε signaling in both the NAc and CeA is a major contributor to binge alcohol drinking and to the genetic propensity to consume excessive amounts of alcohol.

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