Protein kinase C delta stimulates antigen presentation by Class II MHC in murine dendritic cells

Michael Majewski, Tina O. Bose, Fenna C.M. Sillé, Annette M. Pollington, Edda Fiebiger, Marianne Boes

Research output: Contribution to journalArticle

Abstract

Maturation of dendritic cells (DCs) regulates protein sorting in endosomal compartments to promote the surface expression of molecules involved in T cell activation. MHC Class II complexes are mobilized to the surface via intracellular effector molecules that remain largely unknown. We here show that protein kinase C (PKC) stimulates Class II antigen surface expression, using knock-in mice that express a Class II-green fluorescent protein fusion protein as a read out. Selective inhibition of PKCδ counteracts the ability of DCs to stimulate Class II MHC-restricted antigen-specific T cells. Activation of PKC does not affect antigen uptake, peptide loading and surface display of Class I MHC and transferrin receptor in DCs. We show that activation-induced Class II MHC surface expression is dependent on activation of PKCδ and conclude that this event is pivotal for optimal CD4 T cell activation.

Original languageEnglish (US)
Pages (from-to)719-732
Number of pages14
JournalInternational Immunology
Volume19
Issue number6
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Keywords

  • Antigen presentation/processing
  • Antigens/peptides/epitopes
  • Dendritic cells
  • Rodent

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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