The overexpression of c-myc frequently accompanies the relapse of small cell lung cancer (SCLC) cells and contributes to the poor prognosis of this tumor. In this study, we confirm that transfected c-myc results in decreased homotypic cell aggregation and increased proliferative capacity of SCLC cells when nutrient conditions are adequate. We also find that c-myc contributes to apoptosis when cells are nutrient depleted, and flow cytometry suggests that this enhanced apoptosis is associated with a failure to halt cell cycling, consistent with the experience in other cell types. We previously found that protein kinase C-β (PKC-β) expression in NCI H209 (209) SCLC cells increases markedly with c-myc transfection (L. F. Barr et al., Cancer Res., 51: 5514-5519, 1991), and we hypothesized that PKC-β may mediate some of the effects of c-myc in these cells. We test this hypothesis by transfection of rat PKC-β1 and bovine PKC-β2 isoforms into 209 cells before and after transfection with c-myc. PKC-β1 transfection has no effect on these cells. However, PKC-β2 expression has distinct phenotypic consequences. In the parental cells, PKC-β2 expression results in increased homotypic cell aggregation and a prolonged doubling time. Furthermore, PKC-β2 expression increases the fraction of these cells in G0-G1. In the cells which express a transfected c-myc gene, PKC-β2 expression improves the survival of cells in low serum by decreasing myc-induced apoptosis. This effect was associated with, and may be mediated by, a selection for cells in the G0-G1 fraction. We postulate that transfection of c-myc into SCLC cells may select for those expressing the PKC-β2 gene because this signal transduction event protects against myc-induced apoptosis.
|Original language||English (US)|
|Number of pages||12|
|Journal||Cell Growth and Differentiation|
|State||Published - Apr 1997|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology