Protein kinase and phosphatase modulation of quail brain GABA(A) and non-NMDA receptors co-expressed in Xenopus oocytes

C. Moon, S. P. Fraser, M. B A Djamgoz

Research output: Contribution to journalArticlepeer-review

Abstract

The GABA(A) receptor and the non-NMDA subtype of the ionotropic glutamate receptor were co-expressed in Xenopus oocytes by injection of quail brain mRNA. The oocytes were treated with various protein kinase (PK) and protein phosphatase (PP) activators and inhibitors and the effects on receptor functioning were monitored. Two phorbol esters, 4-β-phorbol 12-myristate-13-acetate (PMA) and 4-β-phorbol 12,13-dibutyrate (PDBu); the cGMP-dependent PK activators sodium nitroprusside (SNP) and S-nitrosoglutathione (SNOG); and the PP inhibitor okadaic acid (OA) reduced the amplitude of the GABA-induced currents, whilst the PK inhibitor staurosporine potentiated it. In addition, PMA, PDBu, SNP, and OA reduced the desensitization of the GABA-induced response. Identical treatments generally had similar but less pronounced effects on responses generated by kainate (KA) but the desensitization characteristic of the non-NMDA receptor was not affected. None of the treatments had any effect on the reversal potentials of the induced currents. Immunoblots revealed that the oocytes express endogenous PKG and guanylate cyclase. The results are discussed in terms of the molecular structures of GABA(A) and non-NMDA receptors and the potential functional consequences of phosphorylation/dephosphorylation. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalCellular Signalling
Volume12
Issue number2
DOIs
StatePublished - Feb 2000
Externally publishedYes

Keywords

  • cGMP
  • GABA(A) receptor
  • Guanylate cyclase
  • NO
  • Non-NMDA receptor
  • Protein kinase
  • Protein phosphatase
  • Xenopus oocytes

ASJC Scopus subject areas

  • Cell Biology

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