Protein kinase A signalling via CREB controls myogenesis induced by Wnt proteins

Alice E. Chen, David D. Ginty, Chen Ming Fan

Research output: Contribution to journalArticle

Abstract

Select members of the Wnt family of secreted glycoproteins have been implicated in inducing the myogenic determinant genes Pax3, MyoD and Myf5 during mammalian embryogenesis, but the mechanism of induction has not been defined. We describe an unexpected role for protein kinase A (PKA) signalling via CREB in this induction. Using a combination of in vitro explant assays, mutant analysis and gene delivery into mouse embryos cultured ex vivo, we demonstrate that adenylyl cyclase signalling via PKA and its target transcription factor CREB are required for Wnt-directed myogenic gene expression. Wnt proteins can also stimulate CREB-mediated transcription, providing evidence for a Wnt signalling pathway involving PKA and CREB. Our findings raise the possibility that PKA/CREB signalling may also contribute to other Wnt-regulated processes in embryonic patterning, stem cell renewal and cancer.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalNature
Volume433
Issue number7023
DOIs
StatePublished - Jan 20 2005

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Wnt Proteins
Muscle Development
Cyclic AMP-Dependent Protein Kinases
Wnt Signaling Pathway
Embryonic Stem Cells
Adenylyl Cyclases
Genes
Embryonic Development
Glycoproteins
Transcription Factors
Embryonic Structures
Gene Expression
Neoplasms

ASJC Scopus subject areas

  • General

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Protein kinase A signalling via CREB controls myogenesis induced by Wnt proteins. / Chen, Alice E.; Ginty, David D.; Fan, Chen Ming.

In: Nature, Vol. 433, No. 7023, 20.01.2005, p. 317-322.

Research output: Contribution to journalArticle

Chen, Alice E. ; Ginty, David D. ; Fan, Chen Ming. / Protein kinase A signalling via CREB controls myogenesis induced by Wnt proteins. In: Nature. 2005 ; Vol. 433, No. 7023. pp. 317-322.
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