Protein interaction discovery using parallel analysis of translated ORFs (PLATO)

Jian Zhu, Harry Larman, Geng Gao, Romel Somwar, Zijuan Zhang, Uri Laserson, Alberto Ciccia, Natalya Pavlova, George Church, Wei Zhang, Santosh Kesari, Stephen J. Elledge

Research output: Contribution to journalArticle

Abstract

Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of LYN kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib.

Original languageEnglish (US)
Pages (from-to)331-334
Number of pages4
JournalNature Biotechnology
Volume31
Issue number4
DOIs
StatePublished - Apr 2013
Externally publishedYes

Fingerprint

Open Reading Frames
Proteins
Molecules
DNA Sequence Analysis
Ribosomes
Autoantibodies
Libraries
DNA
Phosphotransferases
gefitinib
Dasatinib
In Vitro Techniques

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering

Cite this

Zhu, J., Larman, H., Gao, G., Somwar, R., Zhang, Z., Laserson, U., ... Elledge, S. J. (2013). Protein interaction discovery using parallel analysis of translated ORFs (PLATO). Nature Biotechnology, 31(4), 331-334. https://doi.org/10.1038/nbt.2539

Protein interaction discovery using parallel analysis of translated ORFs (PLATO). / Zhu, Jian; Larman, Harry; Gao, Geng; Somwar, Romel; Zhang, Zijuan; Laserson, Uri; Ciccia, Alberto; Pavlova, Natalya; Church, George; Zhang, Wei; Kesari, Santosh; Elledge, Stephen J.

In: Nature Biotechnology, Vol. 31, No. 4, 04.2013, p. 331-334.

Research output: Contribution to journalArticle

Zhu, J, Larman, H, Gao, G, Somwar, R, Zhang, Z, Laserson, U, Ciccia, A, Pavlova, N, Church, G, Zhang, W, Kesari, S & Elledge, SJ 2013, 'Protein interaction discovery using parallel analysis of translated ORFs (PLATO)', Nature Biotechnology, vol. 31, no. 4, pp. 331-334. https://doi.org/10.1038/nbt.2539
Zhu, Jian ; Larman, Harry ; Gao, Geng ; Somwar, Romel ; Zhang, Zijuan ; Laserson, Uri ; Ciccia, Alberto ; Pavlova, Natalya ; Church, George ; Zhang, Wei ; Kesari, Santosh ; Elledge, Stephen J. / Protein interaction discovery using parallel analysis of translated ORFs (PLATO). In: Nature Biotechnology. 2013 ; Vol. 31, No. 4. pp. 331-334.
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