Protein filter binding

Sarah Kolitz, Jon R. Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol describes a method to monitor the binding of nucleic acid to protein, allowing the determination of the apparent affinity of a nucleic acid-protein interaction.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Pages197-205
Number of pages9
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Binding
  • Nucleic acid-protein interaction
  • Nytran™ SuPerCharge (SPC) membranes
  • Protein filter binding
  • Whatman GF/C filters

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Kolitz, S., & Lorsch, J. R. (2014). Protein filter binding. In Laboratory Methods in Enzymology: Protein Part C (pp. 197-205). (Methods in Enzymology; Vol. 541). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420119-4.00016-1