Protein filter binding

Sarah Kolitz, Jon R. Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol describes a method to monitor the binding of nucleic acid to protein, allowing the determination of the apparent affinity of a nucleic acid-protein interaction.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Pages197-205
Number of pages9
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Binding
  • Nucleic acid-protein interaction
  • Nytran™ SuPerCharge (SPC) membranes
  • Protein filter binding
  • Whatman GF/C filters

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Protein filter binding'. Together they form a unique fingerprint.

Cite this