Protein chip fabrication by capture of nascent polypeptides

Sheng Ce Tao, Heng Zhu

Research output: Contribution to journalArticle

Abstract

The most challenging step in protein microarray fabrication is high-throughput production of proteins. Here we report two similar strategies to fabricate protein chips through capture onto a solid surface of the nascent polypeptides during translation of synthetic or in vitro-transcribed RNAs. Using these approaches, we efficiently fabricated both peptide and protein microarrays at relatively high density. We further demonstrated that such protein chips can be used to analyze protein activity.

Original languageEnglish (US)
Pages (from-to)1253-1254
Number of pages2
JournalNature Biotechnology
Volume24
Issue number10
DOIs
StatePublished - Oct 2006

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Protein Array Analysis
Polypeptides
Proteins
Fabrication
Peptides
Microarrays
RNA
Throughput

ASJC Scopus subject areas

  • Microbiology

Cite this

Protein chip fabrication by capture of nascent polypeptides. / Tao, Sheng Ce; Zhu, Heng.

In: Nature Biotechnology, Vol. 24, No. 10, 10.2006, p. 1253-1254.

Research output: Contribution to journalArticle

Tao, Sheng Ce ; Zhu, Heng. / Protein chip fabrication by capture of nascent polypeptides. In: Nature Biotechnology. 2006 ; Vol. 24, No. 10. pp. 1253-1254.
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