Protein-bound glycogen is linked to tyrosine residues

M. A. Aon, J. A. Curtino

Research output: Contribution to journalArticle

Abstract

Tyrosine-glycogen obtained from retina proteoglycogen by exhaustive proteolytic digestion was radiolabelled with 125I. The 125I-labelled tyrosine-glycogen was degraded by amylolytic digestion to a very small radioactive product, which was identified as iodotyrosine by h.p.l.c. The amylolytic mixture used released glucose and maltose that were α-linked to the phenolic hydroxy group of p-nitrophenol. No free iodotyrosine was found before or after the intact [125I]iodotyrosine-glycogen was subjected to two cycles of the Edman degradation procedure. The linkage between protein and glycogen was alkali-stable. Therefore it is concluded that the protein-bound glycogen was O-glycosidically linked to the phenolic hydroxy group of tyrosine. The amino acid has not been heretofore found to be involved in the linkage of carbohydrates to proteins.

Original languageEnglish (US)
Pages (from-to)269-272
Number of pages4
JournalBiochemical Journal
Volume229
Issue number1
StatePublished - 1985
Externally publishedYes

Fingerprint

Glycogen
Monoiodotyrosine
Tyrosine
Proteins
Digestion
Carbohydrate Conformation
Maltose
Alkalies
Retina
Carbohydrates
Amino Acids
Glucose
Degradation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Aon, M. A., & Curtino, J. A. (1985). Protein-bound glycogen is linked to tyrosine residues. Biochemical Journal, 229(1), 269-272.

Protein-bound glycogen is linked to tyrosine residues. / Aon, M. A.; Curtino, J. A.

In: Biochemical Journal, Vol. 229, No. 1, 1985, p. 269-272.

Research output: Contribution to journalArticle

Aon, MA & Curtino, JA 1985, 'Protein-bound glycogen is linked to tyrosine residues', Biochemical Journal, vol. 229, no. 1, pp. 269-272.
Aon, M. A. ; Curtino, J. A. / Protein-bound glycogen is linked to tyrosine residues. In: Biochemical Journal. 1985 ; Vol. 229, No. 1. pp. 269-272.
@article{a780a98b867048b6b32f45946c131754,
title = "Protein-bound glycogen is linked to tyrosine residues",
abstract = "Tyrosine-glycogen obtained from retina proteoglycogen by exhaustive proteolytic digestion was radiolabelled with 125I. The 125I-labelled tyrosine-glycogen was degraded by amylolytic digestion to a very small radioactive product, which was identified as iodotyrosine by h.p.l.c. The amylolytic mixture used released glucose and maltose that were α-linked to the phenolic hydroxy group of p-nitrophenol. No free iodotyrosine was found before or after the intact [125I]iodotyrosine-glycogen was subjected to two cycles of the Edman degradation procedure. The linkage between protein and glycogen was alkali-stable. Therefore it is concluded that the protein-bound glycogen was O-glycosidically linked to the phenolic hydroxy group of tyrosine. The amino acid has not been heretofore found to be involved in the linkage of carbohydrates to proteins.",
author = "Aon, {M. A.} and Curtino, {J. A.}",
year = "1985",
language = "English (US)",
volume = "229",
pages = "269--272",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Protein-bound glycogen is linked to tyrosine residues

AU - Aon, M. A.

AU - Curtino, J. A.

PY - 1985

Y1 - 1985

N2 - Tyrosine-glycogen obtained from retina proteoglycogen by exhaustive proteolytic digestion was radiolabelled with 125I. The 125I-labelled tyrosine-glycogen was degraded by amylolytic digestion to a very small radioactive product, which was identified as iodotyrosine by h.p.l.c. The amylolytic mixture used released glucose and maltose that were α-linked to the phenolic hydroxy group of p-nitrophenol. No free iodotyrosine was found before or after the intact [125I]iodotyrosine-glycogen was subjected to two cycles of the Edman degradation procedure. The linkage between protein and glycogen was alkali-stable. Therefore it is concluded that the protein-bound glycogen was O-glycosidically linked to the phenolic hydroxy group of tyrosine. The amino acid has not been heretofore found to be involved in the linkage of carbohydrates to proteins.

AB - Tyrosine-glycogen obtained from retina proteoglycogen by exhaustive proteolytic digestion was radiolabelled with 125I. The 125I-labelled tyrosine-glycogen was degraded by amylolytic digestion to a very small radioactive product, which was identified as iodotyrosine by h.p.l.c. The amylolytic mixture used released glucose and maltose that were α-linked to the phenolic hydroxy group of p-nitrophenol. No free iodotyrosine was found before or after the intact [125I]iodotyrosine-glycogen was subjected to two cycles of the Edman degradation procedure. The linkage between protein and glycogen was alkali-stable. Therefore it is concluded that the protein-bound glycogen was O-glycosidically linked to the phenolic hydroxy group of tyrosine. The amino acid has not been heretofore found to be involved in the linkage of carbohydrates to proteins.

UR - http://www.scopus.com/inward/record.url?scp=0021853157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021853157&partnerID=8YFLogxK

M3 - Article

C2 - 2412547

AN - SCOPUS:0021853157

VL - 229

SP - 269

EP - 272

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -