TY - JOUR
T1 - Protein analysis on a proteomic scale
AU - Phizicky, Eric
AU - Bastiaens, Philippe I.H.
AU - Zhu, Heng
AU - Snyder, Michael
AU - Fields, Stanley
N1 - Funding Information:
Acknowledgements We thank T. Davis and E. Grayhack for comments on the manuscript. This work was supported by grants from the National Center for Research Resources and National Human Genome Research Institute of the National Institutes of Health. S.F. is an investigator of the Howard Hughes Medical Institute.
PY - 2003/3/13
Y1 - 2003/3/13
N2 - The long-term challenge of proteomics is enormous: to define the identities, quantities, structures and functions of complete complements of proteins, and to characterize how these properties vary in different cellular contexts. One critical step in tackling this goal is the generation of sets of clones that express a representative of each protein of a proteome in a useful format, followed by the analysis of these sets on a genome-wide basis. Such studies enable genetic, biochemical and cell biological technologies to be applied on a systematic level, leading to the assignment of biochemical activities, the construction of protein arrays, the identification of interactions, and the localization of proteins within cellular compartments.
AB - The long-term challenge of proteomics is enormous: to define the identities, quantities, structures and functions of complete complements of proteins, and to characterize how these properties vary in different cellular contexts. One critical step in tackling this goal is the generation of sets of clones that express a representative of each protein of a proteome in a useful format, followed by the analysis of these sets on a genome-wide basis. Such studies enable genetic, biochemical and cell biological technologies to be applied on a systematic level, leading to the assignment of biochemical activities, the construction of protein arrays, the identification of interactions, and the localization of proteins within cellular compartments.
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U2 - 10.1038/nature01512
DO - 10.1038/nature01512
M3 - Review article
C2 - 12634794
AN - SCOPUS:0037435022
VL - 422
SP - 208
EP - 215
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6928
ER -