Protein acyltransferase DHHC3 regulates breast tumor growth, oxidative stress, and senescence

Chandan Sharma, Hong Xing Wang, Qinglin Li, Konstantin Knoblich, Emily S. Reisenbichler, Andrea Richardson, Martin E. Hemler

Research output: Contribution to journalArticle

Abstract

DHHC-type protein acyltransferases may regulate the localization, stability, and/or activity of their substrates. In this study, we show that the protein palmitoyltransferase DHHC3 is upregulated in malignant and metastatic human breast cancer. Elevated expression of DHHC3 correlated with diminished patient survival in breast cancer and six other human cancer types. ZDHHC3 ablation in human MDA-MB-231 mammary tumor cell xenografts reduced the sizes of both the primary tumor and metastatic lung colonies. Gene array data and fluorescence dye assays documented increased oxidative stress and senescence in ZDHHC3-ablated cells. ZDHHC3-ablated tumors also showed enhanced recruitment of innate immune cells (antitumor macrophages, natural killer cells) associated with clearance of senescent tumors. These antitumor effects were reversed upon reconstitution with wild-type, but not enzyme-active site-deficient DHHC3. Concomitant ablation of the upregulated oxidative stress protein TXNIP substantially negated the effects of ZDHHC3 depletion on oxidative stress and senescence. Diminished DHHC3-dependent palmitoylation of ERGIC3 protein likely played a key role in TXNIP upregulation. In conclusion, DHHC3-mediated protein palmitoylation supports breast tumor growth by modulating cellular oxidative stress and senescence.

Original languageEnglish (US)
Pages (from-to)6880-6890
Number of pages11
JournalCancer Research
Volume77
Issue number24
DOIs
Publication statusPublished - Dec 15 2017
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Sharma, C., Wang, H. X., Li, Q., Knoblich, K., Reisenbichler, E. S., Richardson, A., & Hemler, M. E. (2017). Protein acyltransferase DHHC3 regulates breast tumor growth, oxidative stress, and senescence. Cancer Research, 77(24), 6880-6890. https://doi.org/10.1158/0008-5472.CAN-17-1536