Protein Acetylation Microarray Reveals that NuA4 Controls Key Metabolic Target Regulating Gluconeogenesis

Yu yi Lin, Jin ying Lu, Junmei Zhang, Wendy Walter, Weiwei Dang, Jun Wan, Sheng Ce Tao, Jiang Qian, Yingming Zhao, Jef D. Boeke, Shelley L. Berger, Heng Zhu

Research output: Contribution to journalArticle

Abstract

Histone acetyltransferases (HATs) and histone deacetylases (HDACs) conduct many critical functions through nonhistone substrates in metazoans, but only chromatin-associated nonhistone substrates are known in Saccharomyces cerevisiae. Using yeast proteome microarrays, we identified and validated many nonchromatin substrates of the essential nucleosome acetyltransferase of H4 (NuA4) complex. Among these, acetylation sites (Lys19 and 514) of phosphoenolpyruvate carboxykinase (Pck1p) were determined by tandem mass spectrometry. Acetylation at Lys514 was crucial for enzymatic activity and the ability of yeast cells to grow on nonfermentable carbon sources. Furthermore, Sir2p deacetylated Pck1p both in vitro and in vivo. Loss of Pck1p activity blocked the extension of yeast chronological life span caused by water starvation. In human hepatocellular carcinoma (HepG2) cells, human Pck1 acetylation and glucose production were dependent on TIP60, the human homolog of ESA1. Our findings demonstrate a regulatory function for the NuA4 complex in glucose metabolism and life span by acetylating a critical metabolic enzyme.

Original languageEnglish (US)
Pages (from-to)1073-1084
Number of pages12
JournalCell
Volume136
Issue number6
DOIs
StatePublished - Mar 20 2009

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Acetylation
Protein Array Analysis
Acetyltransferases
Gluconeogenesis
Nucleosomes
Microarrays
Yeast
Yeasts
Histone Acetyltransferases
Glucose
Phosphoenolpyruvate
Proteins
Histone Deacetylases
Substrates
Hep G2 Cells
Proteome
Tandem Mass Spectrometry
Starvation
Chromatin
Saccharomyces cerevisiae

Keywords

  • CELLBIO
  • HUMDISEASE
  • PROTEINS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Protein Acetylation Microarray Reveals that NuA4 Controls Key Metabolic Target Regulating Gluconeogenesis. / Lin, Yu yi; Lu, Jin ying; Zhang, Junmei; Walter, Wendy; Dang, Weiwei; Wan, Jun; Tao, Sheng Ce; Qian, Jiang; Zhao, Yingming; Boeke, Jef D.; Berger, Shelley L.; Zhu, Heng.

In: Cell, Vol. 136, No. 6, 20.03.2009, p. 1073-1084.

Research output: Contribution to journalArticle

Lin, YY, Lu, JY, Zhang, J, Walter, W, Dang, W, Wan, J, Tao, SC, Qian, J, Zhao, Y, Boeke, JD, Berger, SL & Zhu, H 2009, 'Protein Acetylation Microarray Reveals that NuA4 Controls Key Metabolic Target Regulating Gluconeogenesis', Cell, vol. 136, no. 6, pp. 1073-1084. https://doi.org/10.1016/j.cell.2009.01.033
Lin, Yu yi ; Lu, Jin ying ; Zhang, Junmei ; Walter, Wendy ; Dang, Weiwei ; Wan, Jun ; Tao, Sheng Ce ; Qian, Jiang ; Zhao, Yingming ; Boeke, Jef D. ; Berger, Shelley L. ; Zhu, Heng. / Protein Acetylation Microarray Reveals that NuA4 Controls Key Metabolic Target Regulating Gluconeogenesis. In: Cell. 2009 ; Vol. 136, No. 6. pp. 1073-1084.
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