Protein 4.1R-deficient mice are viable but have erythroid membrane skeleton abnormalities

Zheng Tao Shi, Veena Afzal, Barry Coller, Dipti Patel, Joel A. Chasis, Marilyn Parra, Gloria Lee, Chris Paszty, Mary Stevens, Loren Walensky, Luanne L. Peters, Narla Mohandas, Edward Rubin, John G. Conboy

Research output: Contribution to journalArticle

Abstract

A diverse family of protein 4.1R isoforms is encoded by a complex gene on human chromosome 1. Although the prototypical 80-kDa 4.1R in mature erythrocytes is a key component of the erythroid membrane skeleton that regulates erythrocyte morphology and mechanical stability, little is known about 4.1R function in nucleated cells. Using gene knockout technology, we have generated mice with complete deficiency of all 4.1R protein isoforms. These 4.1R-null mice were viable, with moderate hemolytic anemia but no gross abnormalities. Erythrocytes from these mice exhibited abnormal morphology, lowered membrane stability, and reduced expression of other skeletal proteins including spectrin and ankyrin, suggesting that loss of 4.1R compromises membrane skeleton assembly in erythroid progenitors. Platelet morphology and function were essentially normal, indicating that 4.1R deficiency may have less impact on other hematopoietic lineages. Nonerythroid 4.1R expression patterns, viewed using histochemical staining for lacZ reporter activity incorporated into the targeted gene, revealed focal expression in specific neurons in the brain and in select cells of other major organs, challenging the view that 4.1R expression is widespread among nonerythroid cells. The 4.1R knockout mice represent a valuable animal model for exploring 4.1R function in nonerythroid cells and for determining pathophysiological sequelae to 4.1R deficiency.

Original languageEnglish (US)
Pages (from-to)331-340
Number of pages10
JournalJournal of Clinical Investigation
Volume103
Issue number3
DOIs
StatePublished - Feb 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Shi, Z. T., Afzal, V., Coller, B., Patel, D., Chasis, J. A., Parra, M., Lee, G., Paszty, C., Stevens, M., Walensky, L., Peters, L. L., Mohandas, N., Rubin, E., & Conboy, J. G. (1999). Protein 4.1R-deficient mice are viable but have erythroid membrane skeleton abnormalities. Journal of Clinical Investigation, 103(3), 331-340. https://doi.org/10.1172/JCI3858