Protective immunity to cytomegalovirus (CMV) retinitis in AIDS is associated with CMV-specific T cells that express interferon-γ and interleukin-2 and have a CD8+ cell early maturational phenotype

Elizabeth Sinclair, Xuan Tan Qi, Margaret Sharp, Valerie Girling, Chungkee Poon, Mark L Van Natta, Douglas Jabs, Margaret Inokuma, Holden T. Maecker, Barry Bredt, Mark A. Jacobson

Research output: Contribution to journalArticle

Abstract

To determine potential correlates of immune recovery from AIDS-related cytomegalovirus retinitis (CMVR), multiparameter flow cytometry was used to characterize CMV-specific T cells from subjects with CMVR. Individuals with active retinitis were compared with those who had been clinically immunorestored by antiretroviral therapy and had ≥2 years of ophthalmologic follow-up without anti-CMV therapy or retinitis reactivation or progression. In comparison with patients with active retinitis, immunorestored patients had higher circulating CD4+ and CD8+ T cells expressing interleukin-2 and interferon-γ in response to combined CMV pp65 and IE1 peptide pool stimulation. CD4+ T cell responses were predominantly to pp65, whereas CD8+ T cell responses were predominantly to IE. Immunorestored patients, compared with patients with active retinitis, had increased levels of circulating CMV-specific CD8+ T cells with "early" (CD27+CD28+CD45RA+, CD27 +CD28+CD45RA-) and "intermediate" (CD27-CD28+CD45RA-) phenotypes. Recovery from AIDS-related CMVR after the initiation of antiretroviral therapy may be mediated by CMV-specific CD4+ and CD8+ T cells capable of promoting antigen-specific CD8+ T cell proliferation.

Original languageEnglish (US)
Pages (from-to)1537-1546
Number of pages10
JournalJournal of Infectious Diseases
Volume194
Issue number11
DOIs
StatePublished - Dec 1 2006

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Cytomegalovirus Retinitis
Cytomegalovirus
Interferons
Interleukin-2
Immunity
Acquired Immunodeficiency Syndrome
Retinitis
T-Lymphocytes
Phenotype
CD8 Antigens
Flow Cytometry
Therapeutics
Cell Proliferation
Peptides

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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Protective immunity to cytomegalovirus (CMV) retinitis in AIDS is associated with CMV-specific T cells that express interferon-γ and interleukin-2 and have a CD8+ cell early maturational phenotype. / Sinclair, Elizabeth; Qi, Xuan Tan; Sharp, Margaret; Girling, Valerie; Poon, Chungkee; Van Natta, Mark L; Jabs, Douglas; Inokuma, Margaret; Maecker, Holden T.; Bredt, Barry; Jacobson, Mark A.

In: Journal of Infectious Diseases, Vol. 194, No. 11, 01.12.2006, p. 1537-1546.

Research output: Contribution to journalArticle

Sinclair, Elizabeth ; Qi, Xuan Tan ; Sharp, Margaret ; Girling, Valerie ; Poon, Chungkee ; Van Natta, Mark L ; Jabs, Douglas ; Inokuma, Margaret ; Maecker, Holden T. ; Bredt, Barry ; Jacobson, Mark A. / Protective immunity to cytomegalovirus (CMV) retinitis in AIDS is associated with CMV-specific T cells that express interferon-γ and interleukin-2 and have a CD8+ cell early maturational phenotype. In: Journal of Infectious Diseases. 2006 ; Vol. 194, No. 11. pp. 1537-1546.
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