Protective Effects of Luteolin against Amyloid β25-35-induced Toxicity on Rat Cerebral Microvascular Endothelial Cells

Rui LIU, Xi LAN, Jian YING, Guan Hua DU

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To evaluate the effects of luteolin against amyloid beta-peptide 25-35 (Aβ25-35) on rat cerebral microvascular endothelial cells (CMECs). Methods: CMECs were isolated from Wistar rats at 3-week-old, and randomly divided into 5 groups including control group, Aβ25-35 group, 0.1, 1.0, and 10.0 μmol·L-1 luteolin groups. Cell viability was determined with MTS assay. Intracelluar ROS level and SOD activity were monitored by DCFH-DA and SOD inhibition assay, respectively. Transendothelial electrical resistance was measured using an EVOM resistance meter. γ-Glutamyl transpeptidase and alkaline phosphatase activities were detected using activity assay kits. Levels of TXA2 and PGI2 in culture medium were measured as their stable metabolites, TXB2 and 6-keto-PGF, by ELISA. Results: Luteolin attenuated Aβ25-35-induced toxicity at 0.1, 1.0, and 10 μmol·L-1, inhibited intracellular ROS generation, and increased SOD activity at 1.0 and 10 μmol·L-1. Luteolin was also found to preserve CMECs barrier function, involving the alleviation of TEER reduction, the increase of characteristic enzymatic activity, and regulation of TXA2 and PGI2 secretion. Conclusion: Luteolin had the ability to protect rat CMECs against Aβ25-35-induced toxicity.

Original languageEnglish (US)
Pages (from-to)223-227
Number of pages5
JournalChinese Journal of Natural Medicines
Volume8
Issue number3
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Amyloid beta-peptide
  • Luteolin
  • Microvascular endothelial cells
  • Transendothelial electrical resistance

ASJC Scopus subject areas

  • Drug Discovery
  • Complementary and alternative medicine

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