Protective effects of inducible HO-1 on oxygen toxicity in rat brain endothelial microvessel cells

Seung Jun Yoo, Neal K. Nakra, Gabriele V. Ronnett, Cheil Moon

Research output: Contribution to journalArticle

Abstract

Background: Reperfusion in ischemia is believed to generate cytotoxic oxidative stress, which mediates reperfusion injury. These stress conditions can initiate lipid peroxidation and damage to proteins, as well as promote DNA strand breaks. As biliverdin and bilirubin produced by heme oxygenase isoform 1 (HO-1) have antioxidant properties, the production of both antioxidants by HO-1 may help increase the resistance of the ischemic brain to oxidative stress. In the present study, the survival effect of HO-1 was confirmed using hemin. Methods: To confirm the roles of HO-1, carbon monoxide, and cyclic guanosine monophosphate further in the antioxidant effect of HO-1 and bilirubin, cells were treated with cycloheximide, desferoxamine, and zinc deuteroporphyrin IX 2,4 bis glycol, respectively. Results: HO-1 itself acted as an antioxidant. Furthermore, iron, rather than carbon monoxide, was involved in the HO-1-mediated survival effect. HO-1 activity was also important in providing bilirubin as an antioxidant. Conclusion: Our results suggested that HO-1 helped to increase the resistance of the ischemic brain to oxidative stress.

Original languageEnglish (US)
Pages (from-to)356-362
Number of pages7
JournalEndocrinology and Metabolism
Volume29
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Heme Oxygenase-1
Microvessels
Protein Isoforms
Endothelial Cells
Oxygen
Brain
Antioxidants
Bilirubin
Oxidative Stress
Carbon Monoxide
Biliverdine
Hemin
DNA Breaks
Cyclic GMP
Cycloheximide
Reperfusion Injury
Lipid Peroxidation
Reperfusion
Ischemia
Iron

Keywords

  • Bilirubin
  • Carbon monoxide
  • Heme
  • Iron
  • Oxygenases

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Protective effects of inducible HO-1 on oxygen toxicity in rat brain endothelial microvessel cells. / Yoo, Seung Jun; Nakra, Neal K.; Ronnett, Gabriele V.; Moon, Cheil.

In: Endocrinology and Metabolism, Vol. 29, No. 3, 2014, p. 356-362.

Research output: Contribution to journalArticle

Yoo, Seung Jun ; Nakra, Neal K. ; Ronnett, Gabriele V. ; Moon, Cheil. / Protective effects of inducible HO-1 on oxygen toxicity in rat brain endothelial microvessel cells. In: Endocrinology and Metabolism. 2014 ; Vol. 29, No. 3. pp. 356-362.
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