Protection of zinc sulfate on acute cerebral ischemia reperfusion injury in rats

Wenzhen Duan, Y. Q. Liang, Y. Z. Tang

Research output: Contribution to journalArticle

Abstract

AIM: Cerebral protection of zinc sulfate was observed on the acute cerebral ischemia reperfusion injury in rats. METHODS: Animal model was produced by occlusion of four vessels. At the same time, free intracellular calcium concentration was measured by using calcium sensitive fluorescent indicator Fura-2/AM in isolated embryonic rat brain cells. RESULTS: Pretreatment with zinc sulfate (10 mg · kg-1, iv) significantly declined calcium accumulation and malondialdehyde content, inhibited brain edema and lactic dehydrogenase releasing from brain tissue, promoted the recovery of EEG activities. ZnSO4 (100~300 μmol · L-1) inhibited the [Ca2+](i) elevation induced by high extracellular potassium (50 mmol · L-1), with an IC50 value of 190 (95% confidence limits: 150~230) μmol · L-1, and blocked the L-glutamate (100 μmol · L-1) stimulated increase of [Ca2+], with an IC50 of 210 (95% confidence limits: 170-250) μmol · L-1. CONCLUSION: Zinc sulfate protected brain tissue from ischemia accumulation and anti-lipid peroxidation.

Original languageEnglish (US)
Pages (from-to)39-42
Number of pages4
JournalChinese Pharmacological Bulletin
Volume13
Issue number1
Publication statusPublished - 1997
Externally publishedYes

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Keywords

  • Calcium
  • Cerebral ischemia
  • Fura 2/AM
  • Lipid peroxide
  • Reperfusion injury
  • Zinc sulfate

ASJC Scopus subject areas

  • Pharmacology

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