Abstract
AIM: Cerebral protection of zinc sulfate was observed on the acute cerebral ischemia reperfusion injury in rats. METHODS: Animal model was produced by occlusion of four vessels. At the same time, free intracellular calcium concentration was measured by using calcium sensitive fluorescent indicator Fura-2/AM in isolated embryonic rat brain cells. RESULTS: Pretreatment with zinc sulfate (10 mg · kg-1, iv) significantly declined calcium accumulation and malondialdehyde content, inhibited brain edema and lactic dehydrogenase releasing from brain tissue, promoted the recovery of EEG activities. ZnSO4 (100~300 μmol · L-1) inhibited the [Ca2+](i) elevation induced by high extracellular potassium (50 mmol · L-1), with an IC50 value of 190 (95% confidence limits: 150~230) μmol · L-1, and blocked the L-glutamate (100 μmol · L-1) stimulated increase of [Ca2+], with an IC50 of 210 (95% confidence limits: 170-250) μmol · L-1. CONCLUSION: Zinc sulfate protected brain tissue from ischemia accumulation and anti-lipid peroxidation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 39-42 |
| Number of pages | 4 |
| Journal | Chinese Pharmacological Bulletin |
| Volume | 13 |
| Issue number | 1 |
| State | Published - Jan 1 1997 |
| Externally published | Yes |
Keywords
- Calcium
- Cerebral ischemia
- Fura 2/AM
- Lipid peroxide
- Reperfusion injury
- Zinc sulfate
ASJC Scopus subject areas
- Pharmacology