Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling

Heribert Wefers; Toru Komai; Paul Talalay; Helmut Sies

doi:10.1016/0014-5793(84)80290-2

Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling

Heribert Wefers, Toru Komai, Paul Talalay, Helmut Sies

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Menadione elicits low-level chemiluminescence (λ >620 nm) associated with redox cycling of the quinone in mouse hepatic postmitochondrial fractions. This photoemission is suppressed when the animals are fed a diet containing the anticarcinogenic antioxidant, 2[3]-(tert-butyl)-4-hydroxyanisole (BHA), which leads to a 13-fold increase in NAD(P)H:quinone reductase (EC 1.6.99.2). Inhibition of the enzyme by dicoumarol completely abolishes the protective effect of BHA treatment and leads to higher chemiluminescence, reaching similar photoemission for BHA-treated and control animals. These findings indicate that the two-electron reduction promoted by quinone reductase prevents redox cycling and that BHA protects against reactive oxygen species by elevating the activity of this enzyme.

Original language	English (US)
Pages (from-to)	63-66
Number of pages	4
Journal	FEBS Letters
Volume	169
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(84)80290-2
State	Published - Apr 9 1984
Externally published	Yes

Keywords

DT-diaphorase
Dicoumarol
Dietary antioxidant
Low-level chemiluminescence
Quinone redox cycling
Reactive oxygen species

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(84)80290-2

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Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling. / Wefers, Heribert; Komai, Toru; Talalay, Paul et al.
In: FEBS Letters, Vol. 169, No. 1, 09.04.1984, p. 63-66.

Research output: Contribution to journal › Article › peer-review

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title = "Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling",

abstract = "Menadione elicits low-level chemiluminescence (λ >620 nm) associated with redox cycling of the quinone in mouse hepatic postmitochondrial fractions. This photoemission is suppressed when the animals are fed a diet containing the anticarcinogenic antioxidant, 2[3]-(tert-butyl)-4-hydroxyanisole (BHA), which leads to a 13-fold increase in NAD(P)H:quinone reductase (EC 1.6.99.2). Inhibition of the enzyme by dicoumarol completely abolishes the protective effect of BHA treatment and leads to higher chemiluminescence, reaching similar photoemission for BHA-treated and control animals. These findings indicate that the two-electron reduction promoted by quinone reductase prevents redox cycling and that BHA protects against reactive oxygen species by elevating the activity of this enzyme.",

keywords = "DT-diaphorase, Dicoumarol, Dietary antioxidant, Low-level chemiluminescence, Quinone redox cycling, Reactive oxygen species",

author = "Heribert Wefers and Toru Komai and Paul Talalay and Helmut Sies",

note = "Funding Information: Supported by Deutsche Forschungsgemein-s&aft, ~hwer~~uktprogr~ ~Mecha~sme~ toxischer W~Tku~~~v{\~a}n Fremdst~ffen3a nd by National Foundation for Cancer Research.T X, was on leavef rom Sankyo Co., Tokyo, Japan.",

year = "1984",

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day = "9",

doi = "10.1016/0014-5793(84)80290-2",

language = "English (US)",

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pages = "63--66",

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T1 - Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling

AU - Wefers, Heribert

AU - Komai, Toru

AU - Talalay, Paul

AU - Sies, Helmut

N1 - Funding Information: Supported by Deutsche Forschungsgemein-s&aft, ~hwer~~uktprogr~ ~Mecha~sme~ toxischer W~Tku~~~vãn Fremdst~ffen3a nd by National Foundation for Cancer Research.T X, was on leavef rom Sankyo Co., Tokyo, Japan.

PY - 1984/4/9

Y1 - 1984/4/9

N2 - Menadione elicits low-level chemiluminescence (λ >620 nm) associated with redox cycling of the quinone in mouse hepatic postmitochondrial fractions. This photoemission is suppressed when the animals are fed a diet containing the anticarcinogenic antioxidant, 2[3]-(tert-butyl)-4-hydroxyanisole (BHA), which leads to a 13-fold increase in NAD(P)H:quinone reductase (EC 1.6.99.2). Inhibition of the enzyme by dicoumarol completely abolishes the protective effect of BHA treatment and leads to higher chemiluminescence, reaching similar photoemission for BHA-treated and control animals. These findings indicate that the two-electron reduction promoted by quinone reductase prevents redox cycling and that BHA protects against reactive oxygen species by elevating the activity of this enzyme.

AB - Menadione elicits low-level chemiluminescence (λ >620 nm) associated with redox cycling of the quinone in mouse hepatic postmitochondrial fractions. This photoemission is suppressed when the animals are fed a diet containing the anticarcinogenic antioxidant, 2[3]-(tert-butyl)-4-hydroxyanisole (BHA), which leads to a 13-fold increase in NAD(P)H:quinone reductase (EC 1.6.99.2). Inhibition of the enzyme by dicoumarol completely abolishes the protective effect of BHA treatment and leads to higher chemiluminescence, reaching similar photoemission for BHA-treated and control animals. These findings indicate that the two-electron reduction promoted by quinone reductase prevents redox cycling and that BHA protects against reactive oxygen species by elevating the activity of this enzyme.

KW - DT-diaphorase

KW - Dicoumarol

KW - Dietary antioxidant

KW - Low-level chemiluminescence

KW - Quinone redox cycling

KW - Reactive oxygen species

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DO - 10.1016/0014-5793(84)80290-2

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JO - FEBS Letters

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ER -

Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling

Abstract

Keywords

ASJC Scopus subject areas

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