Protection against multiple influenza A subtypes by vaccination with highly conserved nucleoprotein

Suzanne L. Epstein, Wing Pui Kong, Julia A. Misplon, Chia Yun Lo, Terrence M. Tumpey, Ling Xu, Gary J. Nabel

Research output: Contribution to journalArticle

Abstract

Influenza epidemic and pandemic strains cannot be predicted with certainty. Current vaccines elicit antibodies effective against specific strains, but new strategies are urgently needed for protection against unexpected strains. DNA vaccines encoding conserved antigens protect animals against diverse subtypes, but their potency needs improvement. We tested DNA prime-recombinant adenoviral boost immunization to nucleoprotein (NP). Strong antibody and T cell responses were induced. Protection against challenge was T cell-dependent and substantially more potent than DNA vaccination alone. Importantly, vaccination protected against lethal challenge with highly pathogenic H5N1 virus. Thus, gene-based vaccination with NP may contribute to protective immunity against diverse influenza viruses through its ability to stimulate cellular immunity.

Original languageEnglish (US)
Pages (from-to)5404-5410
Number of pages7
JournalVaccine
Volume23
Issue number46-47
DOIs
StatePublished - Nov 16 2005
Externally publishedYes

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Keywords

  • Adenovirus vectors
  • Animal models
  • Conserved antigens
  • DNA vaccines
  • H5N1 influenza
  • Heterosubtypic immunity
  • Influenza

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Epstein, S. L., Kong, W. P., Misplon, J. A., Lo, C. Y., Tumpey, T. M., Xu, L., & Nabel, G. J. (2005). Protection against multiple influenza A subtypes by vaccination with highly conserved nucleoprotein. Vaccine, 23(46-47), 5404-5410. https://doi.org/10.1016/j.vaccine.2005.04.047