Proteasome inhibitors block development of Plasmodium spp.

Soren M. Gantt, Joon Mo Myung, Marcelo R.S. Briones, Wei Dong Li, E. J. Corey, Satoshi Omura, Victor Nussenzweig, Photini Sinnis

Research output: Contribution to journalArticle

Abstract

Proteasomes degrade most of the proteins inside eukaryotic cells, including transcription factors and regulators of cell cycle progression. Here we show that nanomolar concentrations of lactacystin, a specific irreversible inhibitor of the 20S proteasome, inhibit development of the exoerythrocytic and erythrocytic stages of the malaria parasite. Although lactacystin-treated Plasmodium berghei sporozoites are still invasive, their development into exoerythrocytic forms (EEF) is inhibited in vitro and in vivo. Erythrocytic schizogony of P. falciparum in vitro is also profoundly inhibited when drug treatment of the synchronized parasites is prior, but not subsequent, to the initiation of DNA synthesis, suggesting that the inhibitory effect of lactacystin is cell cycle specific. Lactacystin reduces P. berghei parasitemia in rats, but the therapeutic index is very low. Along with other studies showing that lactacystin inhibits stage-specific transformation in Trypanosoma and Entamoeba spp., these findings highlight the potential of proteasome inhibitors as drugs for the treatment of diseases caused by protozoan parasites.

Original languageEnglish (US)
Pages (from-to)2731-2738
Number of pages8
JournalAntimicrobial agents and chemotherapy
Volume42
Issue number10
DOIs
StatePublished - Oct 1998
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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  • Cite this

    Gantt, S. M., Myung, J. M., Briones, M. R. S., Li, W. D., Corey, E. J., Omura, S., Nussenzweig, V., & Sinnis, P. (1998). Proteasome inhibitors block development of Plasmodium spp. Antimicrobial agents and chemotherapy, 42(10), 2731-2738. https://doi.org/10.1128/aac.42.10.2731