Proteasome biology is compromised in white matter after asphyxic cardiac arrest in neonatal piglets

Polan T. Santos, Caitlin E. O’brien, May W. Chen, C. Danielle Hopkins, Shawn Adams, Ewa Kulikowicz, Rashmi Singh, Raymond C. Koehler, Lee J. Martin, Jennifer K. Lee

Research output: Contribution to journalArticle

Abstract

Background-—Neurological deficits in hypoxic-ischemic encephalopathy, even with therapeutic hypothermia, are partially attributed to white matter injury. We theorized that proteasome insufficiency contributes to white matter injury. Methods and Results-—Neonatal piglets received hypoxia-ischemia (HI) or sham procedure with normothermia, hypothermia, or hypothermia+rewarming. Some received a proteasome activator drug (oleuropein) or white matter–targeted, virus-mediated proteasome knockdown. We measured myelin oligodendrocyte glycoprotein, proteasome subunit 20S (P20S), proteasome activity, and carbonylated and ubiquitinated protein levels in white matter and cerebral cortex. HI reduced myelin oligodendrocyte glycoprotein levels regardless of temperature, and myelin oligodendrocyte glycoprotein loss was associated with increased ubiquitinated and carbonylated protein levels. Ubiquitinated and carbonyl-damaged proteins increased in white matter 29 hours after HI during hypothermia to exceed levels at 6 to 20 hours. In cortex, ubiquitinated proteins decreased. Ubiquitinated and carbonylated protein accumulation coincided with lower P20S levels in white matter; P20S levels also decreased in cerebral cortex. However, proteasome activity in white matter lagged behind that in cortex 29 hours after HI during hypothermia. Systemic oleuropein enhanced white matter P20S and protected the myelin, whereas proteasome knockdown exacerbated myelin oligodendrocyte glycoprotein loss and ubiquitinated protein accumulation after HI. At the cellular level, temperature and HI interactively affected macroglial P20S enrichment in subcortical white matter. Rewarming alone increased macroglial P20S immunoreactivity, but this increase was blocked by HI. Conclusions-—Oxidized and ubiquitinated proteins accumulate with HI-induced white matter injury. Proteasome insufficiency may drive this injury. Hypothermia did not prevent myelin damage, protect the proteasome, or preserve oxidized and ubiquitinated protein clearance after HI.

Original languageEnglish (US)
Article numbere009415
JournalJournal of the American Heart Association
Volume7
Issue number20
DOIs
StatePublished - Oct 1 2018

Fingerprint

Proteasome Endopeptidase Complex
Heart Arrest
Ubiquitinated Proteins
Ischemia
Myelin-Oligodendrocyte Glycoprotein
Hypothermia
Rewarming
Wounds and Injuries
Myelin Sheath
White Matter
Cerebral Cortex
Brain Hypoxia-Ischemia
Hypoxia
Induced Hypothermia
Temperature

Keywords

  • Hypothermia
  • Hypoxia
  • Neonatal ischemia
  • Oxidative stress
  • White matter disease

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Proteasome biology is compromised in white matter after asphyxic cardiac arrest in neonatal piglets. / Santos, Polan T.; O’brien, Caitlin E.; Chen, May W.; Hopkins, C. Danielle; Adams, Shawn; Kulikowicz, Ewa; Singh, Rashmi; Koehler, Raymond C.; Martin, Lee J.; Lee, Jennifer K.

In: Journal of the American Heart Association, Vol. 7, No. 20, e009415, 01.10.2018.

Research output: Contribution to journalArticle

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abstract = "Background-—Neurological deficits in hypoxic-ischemic encephalopathy, even with therapeutic hypothermia, are partially attributed to white matter injury. We theorized that proteasome insufficiency contributes to white matter injury. Methods and Results-—Neonatal piglets received hypoxia-ischemia (HI) or sham procedure with normothermia, hypothermia, or hypothermia+rewarming. Some received a proteasome activator drug (oleuropein) or white matter–targeted, virus-mediated proteasome knockdown. We measured myelin oligodendrocyte glycoprotein, proteasome subunit 20S (P20S), proteasome activity, and carbonylated and ubiquitinated protein levels in white matter and cerebral cortex. HI reduced myelin oligodendrocyte glycoprotein levels regardless of temperature, and myelin oligodendrocyte glycoprotein loss was associated with increased ubiquitinated and carbonylated protein levels. Ubiquitinated and carbonyl-damaged proteins increased in white matter 29 hours after HI during hypothermia to exceed levels at 6 to 20 hours. In cortex, ubiquitinated proteins decreased. Ubiquitinated and carbonylated protein accumulation coincided with lower P20S levels in white matter; P20S levels also decreased in cerebral cortex. However, proteasome activity in white matter lagged behind that in cortex 29 hours after HI during hypothermia. Systemic oleuropein enhanced white matter P20S and protected the myelin, whereas proteasome knockdown exacerbated myelin oligodendrocyte glycoprotein loss and ubiquitinated protein accumulation after HI. At the cellular level, temperature and HI interactively affected macroglial P20S enrichment in subcortical white matter. Rewarming alone increased macroglial P20S immunoreactivity, but this increase was blocked by HI. Conclusions-—Oxidized and ubiquitinated proteins accumulate with HI-induced white matter injury. Proteasome insufficiency may drive this injury. Hypothermia did not prevent myelin damage, protect the proteasome, or preserve oxidized and ubiquitinated protein clearance after HI.",
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T1 - Proteasome biology is compromised in white matter after asphyxic cardiac arrest in neonatal piglets

AU - Santos, Polan T.

AU - O’brien, Caitlin E.

AU - Chen, May W.

AU - Hopkins, C. Danielle

AU - Adams, Shawn

AU - Kulikowicz, Ewa

AU - Singh, Rashmi

AU - Koehler, Raymond C.

AU - Martin, Lee J.

AU - Lee, Jennifer K.

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AB - Background-—Neurological deficits in hypoxic-ischemic encephalopathy, even with therapeutic hypothermia, are partially attributed to white matter injury. We theorized that proteasome insufficiency contributes to white matter injury. Methods and Results-—Neonatal piglets received hypoxia-ischemia (HI) or sham procedure with normothermia, hypothermia, or hypothermia+rewarming. Some received a proteasome activator drug (oleuropein) or white matter–targeted, virus-mediated proteasome knockdown. We measured myelin oligodendrocyte glycoprotein, proteasome subunit 20S (P20S), proteasome activity, and carbonylated and ubiquitinated protein levels in white matter and cerebral cortex. HI reduced myelin oligodendrocyte glycoprotein levels regardless of temperature, and myelin oligodendrocyte glycoprotein loss was associated with increased ubiquitinated and carbonylated protein levels. Ubiquitinated and carbonyl-damaged proteins increased in white matter 29 hours after HI during hypothermia to exceed levels at 6 to 20 hours. In cortex, ubiquitinated proteins decreased. Ubiquitinated and carbonylated protein accumulation coincided with lower P20S levels in white matter; P20S levels also decreased in cerebral cortex. However, proteasome activity in white matter lagged behind that in cortex 29 hours after HI during hypothermia. Systemic oleuropein enhanced white matter P20S and protected the myelin, whereas proteasome knockdown exacerbated myelin oligodendrocyte glycoprotein loss and ubiquitinated protein accumulation after HI. At the cellular level, temperature and HI interactively affected macroglial P20S enrichment in subcortical white matter. Rewarming alone increased macroglial P20S immunoreactivity, but this increase was blocked by HI. Conclusions-—Oxidized and ubiquitinated proteins accumulate with HI-induced white matter injury. Proteasome insufficiency may drive this injury. Hypothermia did not prevent myelin damage, protect the proteasome, or preserve oxidized and ubiquitinated protein clearance after HI.

KW - Hypothermia

KW - Hypoxia

KW - Neonatal ischemia

KW - Oxidative stress

KW - White matter disease

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