Proteasomal activity modulates TGF-β signaling in a gene-specific manner

Fan Zhang, Mia Mönkkönen, Stina Roth, Marikki Laiho

Research output: Contribution to journalArticle

Abstract

Transforming growth factor-β (TGF-β) signaling relies on Smad-signaling pathway controlled in part by the proteasome. Here we demonstrate that inhibition of the proteasome function in mink epithelial cells accumulates both positive and negative modulators of TGF-β signaling, phospho-Smad2 and SnoN. Inhibition of the proteasome led to abrogation of TGF-β target gene regulation in a gene-specific manner. While regulation of p15Ink4b and myc by TGF-β are lost, PAI-1 induction, previously shown to occur in a Smad3-dependent manner, was not affected by treatment of the cells with the proteasomal inhibitor MG132. The results suggest that proteasomal activity is required for TGF-β signaling in a gene-specific manner.

Original languageEnglish (US)
Pages (from-to)58-62
Number of pages5
JournalFEBS Letters
Volume527
Issue number1-3
DOIs
StatePublished - Sep 11 2002
Externally publishedYes

Keywords

  • Proteasome
  • Signaling
  • Smad
  • SnoN
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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