TY - JOUR
T1 - Protease-facilitated transfer of high-molecular-weight proteins during electrotransfer to nitrocellulose
AU - Gibson, Wade
N1 - Funding Information:
I thank Drs. N. Burnett, G. Cohen, N. Fraiser, and D. Kiehart for useful discussions concerning electro-transfer of proteins to nitrocellulose. This work was aided by Research Grants AI 137 18 and AI 16959 from the National Institute for Allergy and Infectious Diseases.
PY - 1981/11/15
Y1 - 1981/11/15
N2 - A procedure is described to enhance the electrophoretic transfer of high-molecular-weight proteins from polyacrylamide gels onto nitrocellulose. The technique takes advantage of in situ proteolysis to reduce the size of large proteins. No apparent loss of low-molecular-weight protein bands was observed, and transferred proteins remained immunoreactive.
AB - A procedure is described to enhance the electrophoretic transfer of high-molecular-weight proteins from polyacrylamide gels onto nitrocellulose. The technique takes advantage of in situ proteolysis to reduce the size of large proteins. No apparent loss of low-molecular-weight protein bands was observed, and transferred proteins remained immunoreactive.
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U2 - 10.1016/0003-2697(81)90147-0
DO - 10.1016/0003-2697(81)90147-0
M3 - Article
C2 - 7039405
AN - SCOPUS:0019882091
VL - 118
SP - 1
EP - 3
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -