Protease-facilitated transfer of high-molecular-weight proteins during electrotransfer to nitrocellulose

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Abstract

A procedure is described to enhance the electrophoretic transfer of high-molecular-weight proteins from polyacrylamide gels onto nitrocellulose. The technique takes advantage of in situ proteolysis to reduce the size of large proteins. No apparent loss of low-molecular-weight protein bands was observed, and transferred proteins remained immunoreactive.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalAnalytical biochemistry
Volume118
Issue number1
DOIs
StatePublished - Nov 15 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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