Prostate-specific membrane antigen (Psma)-targeted photodynamic therapy enhances the delivery of PSMA-targeted magnetic nanoparticles to psma-expressing prostate tumors

Ethel Ngen, Ying Chen, Babak Behnam Azad, Srikanth Boinapally, Desmond Jacob, Ala Lisok, Chentian Shen, Mir S. Hossain, Jiefu Jin, Zaver M. Bhujwalla, Martin G. Pomper, Sangeeta R. Banerjee

Research output: Contribution to journalArticlepeer-review

Abstract

Enhanced vascular permeability in tumors plays an essential role in nanoparticle delivery. Prostate-specific membrane antigen (PSMA) is overexpressed on the epithelium of aggressive prostate cancers (PCs). Here, we evaluated the feasibility of increasing the delivery of PSMA-targeted magnetic nanoparticles (MNPs) to tumors by enhancing vascular permeability in PSMA(+) PC tumors with PSMA-targeted photodynamic therapy (PDT). Method: PSMA(+) PC3 PIP tumor-bearing mice were given a low-molecular-weight PSMA-targeted photosensitizer and treated with fluorescence image-guided PDT, 4 h after. The mice were then given a PSMA-targeted MNP immediately after PDT and monitored with fluorescence imaging and T2-weighted magnetic resonance imaging (T2-W MRI) 18 h, 42 h, and 66 h after MNP administration. Untreated PSMA(+) PC3 PIP tumor-bearing mice were used as negative controls. Results: An 8-fold increase in the delivery of the PSMA-targeted MNPs was detected using T2-W MRI in the pretreated tumors 42 h after PDT, compared to untreated tumors. Additionally, T2-W MRIs revealed enhanced peripheral intra-tumoral delivery of the PSMA-targeted MNPs. That finding is in keeping with two-photon microscopy, which revealed higher vascular densities at the tumor periphery. Conclusion: These results suggest that PSMA-targeted PDT enhances the delivery of PSMA-targeted MNPs to PSMA(+) tumors by enhancing the vascular permeability of the tumors.

Original languageEnglish (US)
Pages (from-to)182-196
Number of pages15
JournalNanotheranostics
Volume5
Issue number2
DOIs
StatePublished - 2021

Keywords

  • Enhanced permeability and retention (EPR) effect
  • Magnetic nanoparticle delivery
  • Magnetic resonance imaging (MRI)
  • Photodynamic therapy (PDT)
  • Prostate cancer

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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