Prostaglandin E 2 promotes the nuclear accumulation of lymphoid enhancer factor-1 in poorly differentiated breast cancer cells

Ioannis Stasinopoulos, Tiffany Greenwood, Kristine Glunde, Zaver M Bhujwalla

Research output: Contribution to journalArticle

Abstract

Products of the COX reaction are frequently elevated in solid tumors and their roles in the malignant phenotype have been extensively investigated. We have shown that COX-2 is essential for the growth of MDA-MB-231 cells in the fat pad of SCID mice and for their extrapulmonary colonization following injection in the tail vein of SCID mice. The molecular changes that follow shRNA-mediated silencing of COX-2 include a significant downregulation of LEF-1, a transcription factor normally activated during development following the Wnt-induced nuclear translocation of β-catenin. We also report that COX-2-silenced cells have reduced nuclear accumulation of LEF-1 protein and that the COX-2 product PGE 2 partially restored nuclear LEF-1 expression in COX-2-silenced cells. Further, we demonstrate that, like parental COX-2 containing MDA-MB-231 cells, COX-2-silenced cells maintain nuclear localization of β-catenin.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalProstaglandins and Other Lipid Mediators
Volume99
Issue number1-2
DOIs
Publication statusPublished - Oct 2012

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Keywords

  • β-Catenin
  • Breast cancer
  • COX-2
  • Cyclooxygenase-2
  • Inflammation
  • LEF-1
  • Lymphoid enhancer factor-1
  • PGE
  • Prostaglandin E
  • Wnt

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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