Prostaglandin D2 and histamine during the immediate and the late-phase components of allergic cutaneous responses

Marek M. Pienkowski, N. Franklin Adkinson, Marshall Plaut, Philip S. Norman, Lawrence M. Lichtenstein

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


With a skin blister technique in which the mediators generated by the trauma of forming the blister are allowed to subside, we have collected human interstitial skin fluid during the course of allergic reactions to ragweed, and measured levels of histamine and prostaglandin D2 (PGD2). Of 18 ragweed-allergic individuals tested, 11 developed both an immediate and a late-phase reaction (LPR) with fivefold-elevated levels of histamine (40 ng/ml) at 30 minutes and a peak level of PGD2 (6.5 ng/ml) later at 2 1 2 hours after ragweed challenge. The other seven allergic individuals had immediate reactions without an LPR lesion and demonstrated somewhat smaller elevations of histamine (25 ng/ml) but much lower levels of PGD2 (1.6 ng/ml; p < 0.05). The time course of appearance of these mediators was identical in both groups of patients. The fluids from unchallenged blisters of allergic and nonallergic patients and the fluids of nonallergic patients challenged with ragweed had similar levels of histamine, at the lower limit of detection, and undetectable PGD2 levels. The peak levels of PGD2 in allergic individuals correlated with the size of the LPR lesion (p < 0.05). These data suggest that the LPR involves the secondary elaboration of mediators different from mediators responsible for the immediate manifestations of the allergic skin reaction.

Original languageEnglish (US)
Pages (from-to)95-100
Number of pages6
JournalThe Journal of allergy and clinical immunology
Issue number1
StatePublished - Jul 1988
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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