TY - JOUR
T1 - Prostacyclin releases endothelium‐derived relaxing factor and potentiates its action in coronary arteries of the pig
AU - Shimokawa, Hiroaki
AU - Flavahan, Nicholas A.
AU - Lorenz, Robert R.
AU - Vanhoutte, Paul M.
PY - 1988/12
Y1 - 1988/12
N2 - The possible interactions between prostacyclin and endothelium‐derived relaxing factor were examined, in isolated coronary arteries of the pig treated with indomethacin (10−5 m). In organ chamber experiments, prostacyclin caused relaxations, which were potentiated in the presence of the endothelium; the potentiation was abolished by oxyhaemoglobin. In bioassay experiments, prostacyclin caused minimal relaxations of bioassay rings without endothelium; these relaxations were potentiated when the bioassay ring was exposed to basally‐released endothelium‐derived relaxing factor (interaction between prostacyclin and basal endothelium‐derived relaxing factor) and further augmented when the endothelial cells were exposed to the prostanoid (stimulated release of endothelium‐derived relaxing factor). The endothelium‐dependent, but not the direct effects of prostacyclin were augmented by superoxide dismutase plus catalase and abolished by oxyhaemoglobin. Forskolin, a direct activator of adenylate cyclase, caused relaxations of rings without endothelium, which were augmented by the presence of the endothelium. The relaxations induced by prostacyclin or forskolin also had an endothelium‐dependent component in basilar and femoral arteries and in jugular veins of the pig. The endothelium‐dependent actions of prostacyclin probably reflect activation of adenylate cyclase. 1988 British Pharmacological Society
AB - The possible interactions between prostacyclin and endothelium‐derived relaxing factor were examined, in isolated coronary arteries of the pig treated with indomethacin (10−5 m). In organ chamber experiments, prostacyclin caused relaxations, which were potentiated in the presence of the endothelium; the potentiation was abolished by oxyhaemoglobin. In bioassay experiments, prostacyclin caused minimal relaxations of bioassay rings without endothelium; these relaxations were potentiated when the bioassay ring was exposed to basally‐released endothelium‐derived relaxing factor (interaction between prostacyclin and basal endothelium‐derived relaxing factor) and further augmented when the endothelial cells were exposed to the prostanoid (stimulated release of endothelium‐derived relaxing factor). The endothelium‐dependent, but not the direct effects of prostacyclin were augmented by superoxide dismutase plus catalase and abolished by oxyhaemoglobin. Forskolin, a direct activator of adenylate cyclase, caused relaxations of rings without endothelium, which were augmented by the presence of the endothelium. The relaxations induced by prostacyclin or forskolin also had an endothelium‐dependent component in basilar and femoral arteries and in jugular veins of the pig. The endothelium‐dependent actions of prostacyclin probably reflect activation of adenylate cyclase. 1988 British Pharmacological Society
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U2 - 10.1111/j.1476-5381.1988.tb11756.x
DO - 10.1111/j.1476-5381.1988.tb11756.x
M3 - Article
C2 - 3064855
AN - SCOPUS:0024209706
SN - 0007-1188
VL - 95
SP - 1197
EP - 1203
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
IS - 4
ER -