Prospective identification and purification of hair cell and supporting cell progenitors from the embryonic cochlea

Angelika Doetzlhofer, Patricia White, Yun Shain Lee, Andrew Groves, Neil Segil

Research output: Contribution to journalArticle

Abstract

Expression of the cyclin-dependent kinase inhibitor p27Kip1 defines a post-mitotic population of cells in the embryonic mammalian cochlea that constitutes the nascent organ of Corti. Here, we describe techniques to purify these precursors using a transgenic p27/GFP reporter and fluorescence activated cell sorting (FACS). We demonstrate that these cells express other markers of the sensory lineage, such as Sox2, and when placed in dissociated cell culture differentiate as hair cells and supporting cells. The purified sensory progenitors thus obtained provide a means of studying the process of hair cell and supporting cell differentiation in vitro, as well as providing a means of analyzing the molecular and physiological properties of this unique population of cells.

Original languageEnglish (US)
Pages (from-to)282-288
Number of pages7
JournalBrain research
Volume1091
Issue number1
DOIs
StatePublished - May 26 2006

Keywords

  • BAC transgenic reporter
  • Cochlea
  • Embryonic hair cell progenitor
  • FACS purification
  • Organ of Corti
  • p27

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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