Prospective cross-sectional evaluation of the small membrane filtration method for diagnosis of pulmonary tuberculosis

Edward Jones-López, Yukari C Manabe, Moises Palaci, Carol Kayiza, Derek Armstrong, Lydia Nakiyingi, Willy Ssengooba, Mary Gaeddert, Rachel Kubiak, Pedro Almeida, David Alland, Reynaldo Dietze, Moses Joloba, Jerrold J. Ellner, Susan E. Dorman

Research output: Contribution to journalArticle

Abstract

Smear microscopy has suboptimal sensitivity, and there is a need to improve its performance since it is commonly used to diagnose tuberculosis (TB). We prospectively evaluated the diagnostic accuracy of the small membrane filtration (SMF) method, an approach that uses a vacuum manifold and is designed to concentrate bacilli onto a filter that can be examined microscopically. We enrolled hospitalized adults suspected to have pulmonary TB in Kampala, Uganda. We obtained a clinical history and three spontaneously expectorated sputum specimens for smear microscopy (direct, concentrated, and SMF), MGIT (mycobacterial growth indicator tube) 960 and Lowenstein-Jensen (LJ) cultures, and Xpert MTB/RIF testing. We performed per-specimen (primary) and per-patient analyses. From October 2012 to June 2013, we enrolled 212 patients (579 sputum specimens). The participants were mostly female (63.2%), and 81.6% were HIV infected; their median CD4 cell count was 47 cells/μl. Overall, 19.0%, 20.4%, 27.1%, 25.2%, and 25.9% of specimens tested positive by direct smear, concentrated smear, MGIT culture, LJ culture, and Xpert test, respectively. In the per-specimen analysis, the sensitivity of the SMF method (48.5%; 95% confidence interval [CI], 37.4 to 59.6) was lower than those of direct smear (60.9%; 51.4 to 70.5 [P=0.0001]) and concentrated smear (63.3%; 53.6 to 73.1 [P

Original languageEnglish (US)
Pages (from-to)2513-2520
Number of pages8
JournalJournal of Clinical Microbiology
Volume52
Issue number7
DOIs
StatePublished - 2014

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Pulmonary Tuberculosis
Sputum
Membranes
Microscopy
Uganda
CD4 Lymphocyte Count
Growth
Vacuum
Bacillus
Tuberculosis
HIV
Confidence Intervals

ASJC Scopus subject areas

  • Microbiology (medical)
  • Medicine(all)

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Prospective cross-sectional evaluation of the small membrane filtration method for diagnosis of pulmonary tuberculosis. / Jones-López, Edward; Manabe, Yukari C; Palaci, Moises; Kayiza, Carol; Armstrong, Derek; Nakiyingi, Lydia; Ssengooba, Willy; Gaeddert, Mary; Kubiak, Rachel; Almeida, Pedro; Alland, David; Dietze, Reynaldo; Joloba, Moses; Ellner, Jerrold J.; Dorman, Susan E.

In: Journal of Clinical Microbiology, Vol. 52, No. 7, 2014, p. 2513-2520.

Research output: Contribution to journalArticle

Jones-López, E, Manabe, YC, Palaci, M, Kayiza, C, Armstrong, D, Nakiyingi, L, Ssengooba, W, Gaeddert, M, Kubiak, R, Almeida, P, Alland, D, Dietze, R, Joloba, M, Ellner, JJ & Dorman, SE 2014, 'Prospective cross-sectional evaluation of the small membrane filtration method for diagnosis of pulmonary tuberculosis', Journal of Clinical Microbiology, vol. 52, no. 7, pp. 2513-2520. https://doi.org/10.1128/JCM.00642-14
Jones-López, Edward ; Manabe, Yukari C ; Palaci, Moises ; Kayiza, Carol ; Armstrong, Derek ; Nakiyingi, Lydia ; Ssengooba, Willy ; Gaeddert, Mary ; Kubiak, Rachel ; Almeida, Pedro ; Alland, David ; Dietze, Reynaldo ; Joloba, Moses ; Ellner, Jerrold J. ; Dorman, Susan E. / Prospective cross-sectional evaluation of the small membrane filtration method for diagnosis of pulmonary tuberculosis. In: Journal of Clinical Microbiology. 2014 ; Vol. 52, No. 7. pp. 2513-2520.
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AU - Manabe, Yukari C

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AU - Armstrong, Derek

AU - Nakiyingi, Lydia

AU - Ssengooba, Willy

AU - Gaeddert, Mary

AU - Kubiak, Rachel

AU - Almeida, Pedro

AU - Alland, David

AU - Dietze, Reynaldo

AU - Joloba, Moses

AU - Ellner, Jerrold J.

AU - Dorman, Susan E.

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