Propofol and in vivo oxidative stress: Effects of preservative

Research output: Contribution to journalArticle

Abstract

Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. An ischemia-reperfusion model of lung injury in sheep has been studied in which pulmonary and bronchial lung perfusion could be interrupted and restored. The goal of this study was to investigate whether two commercial formulations of propofol and the individual components of the commercial formulations attenuated the oxidative stress produced in this model. Breath ethane and breath carbon monoxide were measured as biomarkers of oxidative stress that occur at reperfusion of ischemic tissue. Data were analyzed by a standard least-squares-fit model. One of the formulations for propofol, which contained the preservative ethylenediaminetetraacetic acid (EDTA), was found to decrease the overall level of oxidative stress in sheep. Furthermore, while several models of severe lung injury demonstrate additional production of reactive oxygen species, our model of ischemia/reperfusion of lung tissue did not.

Original languageEnglish (US)
Article number016003
JournalJournal of Breath Research
Volume3
Issue number1
DOIs
StatePublished - 2009

Fingerprint

Propofol
Oxidative Stress
Reperfusion
Ethane
Lung Injury
Lung
Reactive Oxygen Species
Sheep
Ischemia
Body Weights and Measures
Carbon Monoxide
Least-Squares Analysis
Edetic Acid
Lipid Peroxidation
Perfusion
Biomarkers
Inflammation
Wounds and Injuries

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Propofol and in vivo oxidative stress : Effects of preservative. / Brown, Robert Howard; Wagner, Elizabeth Marie; Cope, Keary A.; Risby, Terence H.

In: Journal of Breath Research, Vol. 3, No. 1, 016003, 2009.

Research output: Contribution to journalArticle

@article{020369cbb2be4c5ea4cfe271574096fb,
title = "Propofol and in vivo oxidative stress: Effects of preservative",
abstract = "Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. An ischemia-reperfusion model of lung injury in sheep has been studied in which pulmonary and bronchial lung perfusion could be interrupted and restored. The goal of this study was to investigate whether two commercial formulations of propofol and the individual components of the commercial formulations attenuated the oxidative stress produced in this model. Breath ethane and breath carbon monoxide were measured as biomarkers of oxidative stress that occur at reperfusion of ischemic tissue. Data were analyzed by a standard least-squares-fit model. One of the formulations for propofol, which contained the preservative ethylenediaminetetraacetic acid (EDTA), was found to decrease the overall level of oxidative stress in sheep. Furthermore, while several models of severe lung injury demonstrate additional production of reactive oxygen species, our model of ischemia/reperfusion of lung tissue did not.",
author = "Brown, {Robert Howard} and Wagner, {Elizabeth Marie} and Cope, {Keary A.} and Risby, {Terence H}",
year = "2009",
doi = "10.1088/1752-7155/3/1/016003",
language = "English (US)",
volume = "3",
journal = "Journal of Breath Research",
issn = "1752-7155",
publisher = "IOP Publishing Ltd.",
number = "1",

}

TY - JOUR

T1 - Propofol and in vivo oxidative stress

T2 - Effects of preservative

AU - Brown, Robert Howard

AU - Wagner, Elizabeth Marie

AU - Cope, Keary A.

AU - Risby, Terence H

PY - 2009

Y1 - 2009

N2 - Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. An ischemia-reperfusion model of lung injury in sheep has been studied in which pulmonary and bronchial lung perfusion could be interrupted and restored. The goal of this study was to investigate whether two commercial formulations of propofol and the individual components of the commercial formulations attenuated the oxidative stress produced in this model. Breath ethane and breath carbon monoxide were measured as biomarkers of oxidative stress that occur at reperfusion of ischemic tissue. Data were analyzed by a standard least-squares-fit model. One of the formulations for propofol, which contained the preservative ethylenediaminetetraacetic acid (EDTA), was found to decrease the overall level of oxidative stress in sheep. Furthermore, while several models of severe lung injury demonstrate additional production of reactive oxygen species, our model of ischemia/reperfusion of lung tissue did not.

AB - Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. An ischemia-reperfusion model of lung injury in sheep has been studied in which pulmonary and bronchial lung perfusion could be interrupted and restored. The goal of this study was to investigate whether two commercial formulations of propofol and the individual components of the commercial formulations attenuated the oxidative stress produced in this model. Breath ethane and breath carbon monoxide were measured as biomarkers of oxidative stress that occur at reperfusion of ischemic tissue. Data were analyzed by a standard least-squares-fit model. One of the formulations for propofol, which contained the preservative ethylenediaminetetraacetic acid (EDTA), was found to decrease the overall level of oxidative stress in sheep. Furthermore, while several models of severe lung injury demonstrate additional production of reactive oxygen species, our model of ischemia/reperfusion of lung tissue did not.

UR - http://www.scopus.com/inward/record.url?scp=68149120243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68149120243&partnerID=8YFLogxK

U2 - 10.1088/1752-7155/3/1/016003

DO - 10.1088/1752-7155/3/1/016003

M3 - Article

C2 - 21383451

AN - SCOPUS:68149120243

VL - 3

JO - Journal of Breath Research

JF - Journal of Breath Research

SN - 1752-7155

IS - 1

M1 - 016003

ER -