Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy

Manu N. Capoor, Filip Ruzicka, Jonathan E. Schmitz, Garth A. James, Tana Machackova, Radim Jancalek, Martin Smrcka, Radim Lipina, Fahad S. Ahmed, Todd F. Alamin, Neel Anand, John C. Baird, Nitin Bhatia, Sibel Demir-Deviren, Robert K. Eastlack, Steve Fisher, Steven R. Garfin, Jaspaul S. Gogia, Ziya L. Gokaslan, Calvin C. KuoYu Po Lee, Konstantinos Mavrommatis, Elleni Michu, Hana Noskova, Assaf Raz, Jiri Sana, A. Nick Shamie, Philip S. Stewart, Jerry L. Stonemetz, Jeffrey C. Wang, Timothy F. Witham, Michael F. Coscia, Christof Birkenmaier, Vincent A. Fischetti, Ondrej Slaby

Research output: Contribution to journalArticle

Abstract

Background In previous studies, Propionibacterium acnes was cultured from intervertebral disc tissue of ~25% of patients undergoing microdiscectomy, suggesting a possible link between chronic bacterial infection and disc degeneration. However, given the prominence of P. acnes as a skin commensal, such analyses often struggled to exclude the alternate possibility that these organisms represent perioperative microbiologic contamination. This investigation seeks to validate P. acnes prevalence in resected disc cultures, while providing microscopic evidence of P. acnes biofilm in the intervertebral discs. Methods Specimens from 368 patients undergoing microdiscectomy for disc herniation were divided into several fragments, one being homogenized, subjected to quantitative anaerobic culture, and assessed for bacterial growth, and a second fragment frozen for additional analyses. Colonies were identified by MALDI-TOF mass spectrometry and P. acnes phylotyping was conducted by multiplex PCR. For a sub-set of specimens, bacteria localization within the disc was assessed by microscopy using confocal laser scanning and FISH. Results Bacteria were cultured from 162 discs (44%), including 119 cases (32.3%) with P. acnes. In 89 cases, P. acnes was cultured exclusively; in 30 cases, it was isolated in combination with other bacteria (primarily coagulase-negative Staphylococcus spp.) Among positive specimens, the median P. acnes bacterial burden was 350 CFU/g (12 - ~20,000 CFU/g). Thirtyeight P. acnes isolates were subjected to molecular sub-typing, identifying 4 of 6 defined phylogroups: IA1, IB, IC, and II. Eight culture-positive specimens were evaluated by fluorescence microscopy and revealed P. acnes in situ. Notably, these bacteria demonstrated a biofilm distribution within the disc matrix. P. acnes bacteria were more prevalent in males than females (39% vs. 23%, p = 0.0013). Conclusions This study confirms that P. acnes is prevalent in herniated disc tissue. Moreover, it provides the first visual evidence of P. acnes biofilms within such specimens, consistent with infection rather than microbiologic contamination.

Original languageEnglish (US)
Article numbere0174518
JournalPloS one
Volume12
Issue number4
DOIs
StatePublished - Mar 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Capoor, M. N., Ruzicka, F., Schmitz, J. E., James, G. A., Machackova, T., Jancalek, R., Smrcka, M., Lipina, R., Ahmed, F. S., Alamin, T. F., Anand, N., Baird, J. C., Bhatia, N., Demir-Deviren, S., Eastlack, R. K., Fisher, S., Garfin, S. R., Gogia, J. S., Gokaslan, Z. L., ... Slaby, O. (2017). Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy. PloS one, 12(4), [e0174518]. https://doi.org/10.1371/journal.pone.0174518