Prophase I mouse oocytes are deficient in the ability to respond to fertilization by decreasing membrane receptivity to sperm and establishing a membrane block to polyspermy

Cassie A. Kryzak, Maia M. Moraine, Diane D. Kyle, Hyo J. Lee, Caelin Cubeñas-Potts, Douglas Robinson, Janice Perry Evans

Research output: Contribution to journalArticle

Abstract

Changes occurring as the prophase I oocyte matures to metaphase II are critical for the acquisition of competence for normal egg activation and early embryogenesis. A prophase I oocyte cannot respond to a fertilizing sperm as a metaphase II egg does, including the ability to prevent polyspermic fertilization. Studies here demonstrate that the competence for the membrane block to polyspermy is deficient in prophase I mouse oocytes. In vitro fertilization experiments using identical insemination conditions result in monospermy in 87% of zona pellucida (ZP)-free metaphase II eggs, while 92% of ZP-free prophase I oocytes have four or more fused sperm. The membrane block is associated with a postfertilization reduction in the capacity to support sperm binding, but this reduction in sperm-binding capacity is both less robust and slower to develop in fertilized prophase I oocytes. Fertilization of oocytes is dependent on the tetraspanin CD9, but little to no release of CD9 from the oocyte membrane is detected, suggesting that release of CD9-containing vesicles is not essential for fertilization. The deficiency in membrane block establishment in prophase I oocytes correlates with abnormalities in two postfertilization cytoskeletal changes: sperm-induced cortical remodeling that results in fertilization cone formation and a postfertilization increase in effective cortical tension. These data indicate that cortical maturation is a component of cytoplasmic maturation during the oocyte-to-egg transition and that the egg cortex has to be appropriately primed and tuned to be responsive to a fertilizing sperm.

Original languageEnglish (US)
Article number44
JournalBiology of Reproduction
Volume89
Issue number2
DOIs
StatePublished - 2013

Fingerprint

Meiotic Prophase I
Aptitude
Fertilization
Oocytes
Spermatozoa
Membranes
Ovum
Metaphase
Zona Pellucida
Mental Competency
Insemination
Fertilization in Vitro
Eggs
Embryonic Development

Keywords

  • Activation competence
  • Block to polyspermy
  • CD9
  • Cytoplasmic maturation
  • Polyspermy

ASJC Scopus subject areas

  • Cell Biology

Cite this

Prophase I mouse oocytes are deficient in the ability to respond to fertilization by decreasing membrane receptivity to sperm and establishing a membrane block to polyspermy. / Kryzak, Cassie A.; Moraine, Maia M.; Kyle, Diane D.; Lee, Hyo J.; Cubeñas-Potts, Caelin; Robinson, Douglas; Evans, Janice Perry.

In: Biology of Reproduction, Vol. 89, No. 2, 44, 2013.

Research output: Contribution to journalArticle

Kryzak, Cassie A. ; Moraine, Maia M. ; Kyle, Diane D. ; Lee, Hyo J. ; Cubeñas-Potts, Caelin ; Robinson, Douglas ; Evans, Janice Perry. / Prophase I mouse oocytes are deficient in the ability to respond to fertilization by decreasing membrane receptivity to sperm and establishing a membrane block to polyspermy. In: Biology of Reproduction. 2013 ; Vol. 89, No. 2.
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