Properties of purified recombinant human polyamine oxidase, PAOh1/SMO

Yanlin Wang, Tracy Murray Stewart, Wendy Devereux, Amy Hacker, Benjamin Frydman, Patrick M. Woster, Robert A Casero

Research output: Contribution to journalArticle

Abstract

The discovery of an inducible oxidase whose apparent substrate preference is spermine indicates that polyamine catabolism is more complex than that originally proposed. To facilitate the study of this enzyme, the purification and characterization of the recombinant human PAOh1/SMO polyamine oxidase are reported. Purified PAOh1/SMO oxidizes both spermine (Km=1.6μM) and N1-acetylspermine (Km=51μM), but does not oxidize spermidine. The purified human enzyme also does not oxidize eight representative antitumor polyamine analogues; however, specific oligamine analogues were found to be potent inhibitors of the oxidation of spermine by PAOh1/SMO. The results of these studies are consistent with the hypothesis that PAOh1/SMO represents a new addition to the polyamine metabolic pathway that may represent a new target for antineoplastic drug development.

Original languageEnglish (US)
Pages (from-to)605-611
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume304
Issue number4
DOIs
StatePublished - May 16 2003

Fingerprint

Spermine
Polyamines
Spermidine
Corrosion inhibitors
Enzymes
Metabolic Networks and Pathways
Antineoplastic Agents
Purification
Oxidoreductases
Oxidation
Substrates
polyamine oxidase

Keywords

  • FAD-dependent
  • HO
  • Polyamines
  • Spermidine/spermine N-acetyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Properties of purified recombinant human polyamine oxidase, PAOh1/SMO. / Wang, Yanlin; Murray Stewart, Tracy; Devereux, Wendy; Hacker, Amy; Frydman, Benjamin; Woster, Patrick M.; Casero, Robert A.

In: Biochemical and Biophysical Research Communications, Vol. 304, No. 4, 16.05.2003, p. 605-611.

Research output: Contribution to journalArticle

Wang, Yanlin ; Murray Stewart, Tracy ; Devereux, Wendy ; Hacker, Amy ; Frydman, Benjamin ; Woster, Patrick M. ; Casero, Robert A. / Properties of purified recombinant human polyamine oxidase, PAOh1/SMO. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 304, No. 4. pp. 605-611.
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