TY - JOUR
T1 - Proneural gene-linked neurogenesis in zebrafish cerebellum
AU - Kani, Shuichi
AU - Bae, Young Ki
AU - Shimizu, Takashi
AU - Tanabe, Koji
AU - Satou, Chie
AU - Parsons, Michael J.
AU - Scott, Ethan
AU - Higashijima, Shin Ichi
AU - Hibi, Masahiko
N1 - Funding Information:
We thank B. Appel for the Tg(olig2:EGFP)vu12 line; S.D. Leach for the Tg(ptf1a:EGFP)jh1 line; H. Baier for comments on the manuscript; K. Bando, S. Fujii, and A. Katsuyama for technical assistance; and the members of the Hibi Laboratory for helpful discussions. This work was supported by a grant from NCC, Korea ( NCC-1010270 to Y.-K., B.) and by a grant from RIKEN (to M.H.).
PY - 2010/7
Y1 - 2010/7
N2 - In mammals, cerebellar neurons are categorized as glutamatergic or GABAergic, and are derived from progenitors that express the proneural genes atoh1 or ptf1a, respectively. In zebrafish, three atoh1 genes, atoh1a, atoh1b, and atoh1c, are expressed in overlapping but distinct expression domains in the upper rhombic lip (URL): ptf1a is expressed exclusively in the ventricular zone (VZ). Using transgenic lines expressing fluorescent proteins under the control of the regulatory elements of atoh1a and ptf1a, we traced the lineages of the cerebellar neurons. The atoh1+ progenitors gave rise not only to granule cells but also to neurons of the anteroventral rhombencephalon. The ptf1a+ progenitors generated Purkinje cells. The olig2+ eurydendroid cells, which are glutamatergic, were derived mostly from ptf1a+ progenitors in the VZ but some originated from the atoh1+ progenitors in the URL. In the adult cerebellum, atoh1a, atoh1b, and atoh1c are expressed in the molecular layer of the valvula cerebelli and of the medial corpus cerebelli, and ptf1a was detected in the VZ. The proneural gene expression patterns coincided with the sites of proliferating neuronal progenitors in the adult cerebellum. Our data indicate that proneural gene-linked neurogenesis is evolutionarily conserved in the cerebellum among vertebrates, and that the continuously generated neurons help remodel neural circuits in the adult zebrafish cerebellum.
AB - In mammals, cerebellar neurons are categorized as glutamatergic or GABAergic, and are derived from progenitors that express the proneural genes atoh1 or ptf1a, respectively. In zebrafish, three atoh1 genes, atoh1a, atoh1b, and atoh1c, are expressed in overlapping but distinct expression domains in the upper rhombic lip (URL): ptf1a is expressed exclusively in the ventricular zone (VZ). Using transgenic lines expressing fluorescent proteins under the control of the regulatory elements of atoh1a and ptf1a, we traced the lineages of the cerebellar neurons. The atoh1+ progenitors gave rise not only to granule cells but also to neurons of the anteroventral rhombencephalon. The ptf1a+ progenitors generated Purkinje cells. The olig2+ eurydendroid cells, which are glutamatergic, were derived mostly from ptf1a+ progenitors in the VZ but some originated from the atoh1+ progenitors in the URL. In the adult cerebellum, atoh1a, atoh1b, and atoh1c are expressed in the molecular layer of the valvula cerebelli and of the medial corpus cerebelli, and ptf1a was detected in the VZ. The proneural gene expression patterns coincided with the sites of proliferating neuronal progenitors in the adult cerebellum. Our data indicate that proneural gene-linked neurogenesis is evolutionarily conserved in the cerebellum among vertebrates, and that the continuously generated neurons help remodel neural circuits in the adult zebrafish cerebellum.
KW - Adult neurogenesis
KW - Cerebellum
KW - GABAergic neuron
KW - Glutamatergic neuron
KW - Hindbrain
KW - Proneural gene
KW - Zebrafish
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U2 - 10.1016/j.ydbio.2010.03.024
DO - 10.1016/j.ydbio.2010.03.024
M3 - Article
C2 - 20388506
AN - SCOPUS:77953699068
SN - 0012-1606
VL - 343
SP - 1
EP - 17
JO - Developmental biology
JF - Developmental biology
IS - 1-2
ER -