TY - JOUR
T1 - Promotion of specific in vitro transcription by excised "TATA" box sequences inserted in a foreign nucleotide environment
AU - Sassone-Corsi, P.
AU - Corden, J.
AU - Kédinger, C.
AU - Chambon, P.
N1 - Funding Information:
We thank J.M. Jeltsch for helpful advice during our sequence work, J.M. Gamier for generous gifts of pBR322 plasmid DNA, K. Dott and D. Schmitt for growing cells and virus, E. Badzinski and B. Boulay for help during preparation of the manuscript. We are particularly grateful to C. Hauss for continuous technical assistance. This investigation was supported by grants from the INSERM (PRC de Genie Genetique), from the CNRS (ATP Biologie Moleculaire du Gene, contract n°. 006520/60), from the "Association pour le Developpement de la Recherche sur le Cancer", from
PY - 1981/8/25
Y1 - 1981/8/25
N2 - We have cloned into plasmid pBR322 a DNA fragment extending from position -32 to position -12 of the adenovirus type 2 major late promoter region (position +1 referring to the cap site). In vitro transcription experiments show that this 21 base pair sequence, which contains the Goldberg-Hogness or "TATA" box, is both necessary and sufficient for specific initiation of transcription by RNA polymerase B (or II). Furthermore, we show that similar sequences, randomly occuring in the bacterial plasmid pBR322, are also recognized by the RNA polymerase B transcription machinery and able to promote specific in vitro transcription. Finally, we discuss the possible importance of the nucleotide sequence of the start region in the actual efficiency of initiation of in vitro transcription.
AB - We have cloned into plasmid pBR322 a DNA fragment extending from position -32 to position -12 of the adenovirus type 2 major late promoter region (position +1 referring to the cap site). In vitro transcription experiments show that this 21 base pair sequence, which contains the Goldberg-Hogness or "TATA" box, is both necessary and sufficient for specific initiation of transcription by RNA polymerase B (or II). Furthermore, we show that similar sequences, randomly occuring in the bacterial plasmid pBR322, are also recognized by the RNA polymerase B transcription machinery and able to promote specific in vitro transcription. Finally, we discuss the possible importance of the nucleotide sequence of the start region in the actual efficiency of initiation of in vitro transcription.
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U2 - 10.1093/nar/9.16.3941
DO - 10.1093/nar/9.16.3941
M3 - Article
C2 - 7301576
AN - SCOPUS:0019501207
SN - 0305-1048
VL - 9
SP - 3941
EP - 3958
JO - Nucleic acids research
JF - Nucleic acids research
IS - 16
ER -