Abstract
A novel stress-inducible metallothionein-like gene from rice, designated as rgMT-1 (rice genomic metallothionein-like gene-1), was isolated and sequenced. From the sequence analysis of its 5′-flanking region, two putative TATA boxes, one CAAT box, and several short sequences homologous to regulatory cis-elements previously reported were identified. Two direct repeats, one 10 bp in length (CAAAATCAAA) and the other 11 bp (GTGAAAATACT), respectively, were also found. By transient GUS (beta-glucuronidase) assay, the expression of GUS, in vitro, was enhanced by the presence of the rgMT-1 intron. The critical region which controls the basal transcription was shown to lie between -73 and -36 upstream of rgMT-1, in which one of the two putative TATA boxes was located. The promoter activity was lost completely when both putative TATA boxes were deleted. This is the first report describing the genomic structure and regulation of a monocotyledonous metal-lothionein-lLke gene critical to the response of stress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-18 |
| Number of pages | 10 |
| Journal | Mitochondrial DNA |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1998 |
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Keywords
- GUS
- intron
- metallothionein
- promoter
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Biochemistry
- Endocrinology
Cite this
Promoter structure and activity of type 1 rice metallothionein-like gene. / Hsieh, Hsing Mei; Huang, P C.
In: Mitochondrial DNA, Vol. 9, No. 1, 1998, p. 9-18.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Promoter structure and activity of type 1 rice metallothionein-like gene
AU - Hsieh, Hsing Mei
AU - Huang, P C
PY - 1998
Y1 - 1998
N2 - A novel stress-inducible metallothionein-like gene from rice, designated as rgMT-1 (rice genomic metallothionein-like gene-1), was isolated and sequenced. From the sequence analysis of its 5′-flanking region, two putative TATA boxes, one CAAT box, and several short sequences homologous to regulatory cis-elements previously reported were identified. Two direct repeats, one 10 bp in length (CAAAATCAAA) and the other 11 bp (GTGAAAATACT), respectively, were also found. By transient GUS (beta-glucuronidase) assay, the expression of GUS, in vitro, was enhanced by the presence of the rgMT-1 intron. The critical region which controls the basal transcription was shown to lie between -73 and -36 upstream of rgMT-1, in which one of the two putative TATA boxes was located. The promoter activity was lost completely when both putative TATA boxes were deleted. This is the first report describing the genomic structure and regulation of a monocotyledonous metal-lothionein-lLke gene critical to the response of stress.
AB - A novel stress-inducible metallothionein-like gene from rice, designated as rgMT-1 (rice genomic metallothionein-like gene-1), was isolated and sequenced. From the sequence analysis of its 5′-flanking region, two putative TATA boxes, one CAAT box, and several short sequences homologous to regulatory cis-elements previously reported were identified. Two direct repeats, one 10 bp in length (CAAAATCAAA) and the other 11 bp (GTGAAAATACT), respectively, were also found. By transient GUS (beta-glucuronidase) assay, the expression of GUS, in vitro, was enhanced by the presence of the rgMT-1 intron. The critical region which controls the basal transcription was shown to lie between -73 and -36 upstream of rgMT-1, in which one of the two putative TATA boxes was located. The promoter activity was lost completely when both putative TATA boxes were deleted. This is the first report describing the genomic structure and regulation of a monocotyledonous metal-lothionein-lLke gene critical to the response of stress.
KW - GUS
KW - intron
KW - metallothionein
KW - promoter
UR - http://www.scopus.com/inward/record.url?scp=0032029057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032029057&partnerID=8YFLogxK
U2 - 10.3109/10425179809050020
DO - 10.3109/10425179809050020
M3 - Article
C2 - 9773271
AN - SCOPUS:0032029057
VL - 9
SP - 9
EP - 18
JO - DNA Sequence - Journal of DNA Sequencing and Mapping
JF - DNA Sequence - Journal of DNA Sequencing and Mapping
SN - 1940-1744
IS - 1
ER -