Promoter structure and activity of type 1 rice metallothionein-like gene

Hsing Mei Hsieh, P C Huang

Research output: Contribution to journalArticle

Abstract

A novel stress-inducible metallothionein-like gene from rice, designated as rgMT-1 (rice genomic metallothionein-like gene-1), was isolated and sequenced. From the sequence analysis of its 5′-flanking region, two putative TATA boxes, one CAAT box, and several short sequences homologous to regulatory cis-elements previously reported were identified. Two direct repeats, one 10 bp in length (CAAAATCAAA) and the other 11 bp (GTGAAAATACT), respectively, were also found. By transient GUS (beta-glucuronidase) assay, the expression of GUS, in vitro, was enhanced by the presence of the rgMT-1 intron. The critical region which controls the basal transcription was shown to lie between -73 and -36 upstream of rgMT-1, in which one of the two putative TATA boxes was located. The promoter activity was lost completely when both putative TATA boxes were deleted. This is the first report describing the genomic structure and regulation of a monocotyledonous metal-lothionein-lLke gene critical to the response of stress.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalMitochondrial DNA
Volume9
Issue number1
DOIs
StatePublished - 1998

Fingerprint

Metallothionein
Genes
TATA Box
Glucuronidase
Nucleic Acid Repetitive Sequences
5' Flanking Region
Transcription
Sequence Homology
Introns
Sequence Analysis
Assays
Metals
Oryza

Keywords

  • GUS
  • intron
  • metallothionein
  • promoter

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Endocrinology

Cite this

Promoter structure and activity of type 1 rice metallothionein-like gene. / Hsieh, Hsing Mei; Huang, P C.

In: Mitochondrial DNA, Vol. 9, No. 1, 1998, p. 9-18.

Research output: Contribution to journalArticle

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