Promoter methylation of TSLC1 and tumor suppression by its gene product in human prostate cancer

Hiroshi Fukuhara; Masami Kuramochi; Takeshi Fukami; Kohtaro Kasahara; Mutsuo Furuhata; Takahiro Nobukuni; Tomoko Maruyama; Kana Isogai; Takao Sekiya; Taro Shuin; Tadaichi Kitamura; Roger H. Reeves; Yoshinori Murakami

doi:10.1111/j.1349-7006.2002.tb01297.x

Promoter methylation of TSLC1 and tumor suppression by its gene product in human prostate cancer

Hiroshi Fukuhara, Masami Kuramochi, Takeshi Fukami, Kohtaro Kasahara, Mutsuo Furuhata, Takahiro Nobukuni, Tomoko Maruyama, Kana Isogai, Takao Sekiya, Taro Shuin, Tadaichi Kitamura, Roger H. Reeves, Yoshinori Murakami

School of Medicine

Research output: Contribution to journal › Article › peer-review

94 Scopus citations

Abstract

We recently identified TSLC1, a tumor suppressor gene in human lung cancer. Gene silencing by promoter methylation has been observed frequently in adenocarcinoma of the lung, liver, and pancreas. Here, we demonstrate that TSLC1 expression is also absent or markedly reduced in 3 of 4 prostate cancer cell lines. Promoter sequences of TSLC1 were heavily methylated in PPC-1 cells that lacked TSLC1 expression, supporting the idea that promoter methylation is strongly correlated with complete loss of gene expression. Promoter sequences of TSLC1 were also methylated significantly in 7 of 22 (32%) primary prostate cancers. Hypermethylation of the promoter occurred not only in advanced tumors, but also in relatively early-stage tumors. Restoration of TSLC1 expression substantially suppressed tumor formation of PPC-1 cells in nude mice. These findings indicate that alteration of TSLC1 is involved in prostate cancer.

Original language	English (US)
Pages (from-to)	605-609
Number of pages	5
Journal	Japanese Journal of Cancer Research
Volume	93
Issue number	6
DOIs	https://doi.org/10.1111/j.1349-7006.2002.tb01297.x
State	Published - 2002

Keywords

Promoter methylation
Prostate cancer
Suppresson of tumorigenicity
TSLC1
Tumor suppressor gene

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1111/j.1349-7006.2002.tb01297.x

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Fukuhara, H., Kuramochi, M., Fukami, T., Kasahara, K., Furuhata, M., Nobukuni, T., Maruyama, T., Isogai, K., Sekiya, T., Shuin, T., Kitamura, T., Reeves, R. H., & Murakami, Y. (2002). Promoter methylation of TSLC1 and tumor suppression by its gene product in human prostate cancer. Japanese Journal of Cancer Research, 93(6), 605-609. https://doi.org/10.1111/j.1349-7006.2002.tb01297.x

Fukuhara, H, Kuramochi, M, Fukami, T, Kasahara, K, Furuhata, M, Nobukuni, T, Maruyama, T, Isogai, K, Sekiya, T, Shuin, T, Kitamura, T, Reeves, RH & Murakami, Y 2002, 'Promoter methylation of TSLC1 and tumor suppression by its gene product in human prostate cancer', Japanese Journal of Cancer Research, vol. 93, no. 6, pp. 605-609. https://doi.org/10.1111/j.1349-7006.2002.tb01297.x

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abstract = "We recently identified TSLC1, a tumor suppressor gene in human lung cancer. Gene silencing by promoter methylation has been observed frequently in adenocarcinoma of the lung, liver, and pancreas. Here, we demonstrate that TSLC1 expression is also absent or markedly reduced in 3 of 4 prostate cancer cell lines. Promoter sequences of TSLC1 were heavily methylated in PPC-1 cells that lacked TSLC1 expression, supporting the idea that promoter methylation is strongly correlated with complete loss of gene expression. Promoter sequences of TSLC1 were also methylated significantly in 7 of 22 (32%) primary prostate cancers. Hypermethylation of the promoter occurred not only in advanced tumors, but also in relatively early-stage tumors. Restoration of TSLC1 expression substantially suppressed tumor formation of PPC-1 cells in nude mice. These findings indicate that alteration of TSLC1 is involved in prostate cancer.",

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AU - Furuhata, Mutsuo

AU - Nobukuni, Takahiro

AU - Maruyama, Tomoko

AU - Isogai, Kana

AU - Sekiya, Takao

AU - Shuin, Taro

AU - Kitamura, Tadaichi

AU - Reeves, Roger H.

AU - Murakami, Yoshinori

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AB - We recently identified TSLC1, a tumor suppressor gene in human lung cancer. Gene silencing by promoter methylation has been observed frequently in adenocarcinoma of the lung, liver, and pancreas. Here, we demonstrate that TSLC1 expression is also absent or markedly reduced in 3 of 4 prostate cancer cell lines. Promoter sequences of TSLC1 were heavily methylated in PPC-1 cells that lacked TSLC1 expression, supporting the idea that promoter methylation is strongly correlated with complete loss of gene expression. Promoter sequences of TSLC1 were also methylated significantly in 7 of 22 (32%) primary prostate cancers. Hypermethylation of the promoter occurred not only in advanced tumors, but also in relatively early-stage tumors. Restoration of TSLC1 expression substantially suppressed tumor formation of PPC-1 cells in nude mice. These findings indicate that alteration of TSLC1 is involved in prostate cancer.

KW - Promoter methylation

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KW - Suppresson of tumorigenicity

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Promoter methylation of TSLC1 and tumor suppression by its gene product in human prostate cancer

Abstract

Keywords

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