Promoter methylation of TSLC1 and tumor suppression by its gene product in human prostate cancer

Hiroshi Fukuhara, Masami Kuramochi, Takeshi Fukami, Kohtaro Kasahara, Mutsuo Furuhata, Takahiro Nobukuni, Tomoko Maruyama, Kana Isogai, Takao Sekiya, Taro Shuin, Tadaichi Kitamura, Roger H. Reeves, Yoshinori Murakami

Research output: Contribution to journalArticle

Abstract

We recently identified TSLC1, a tumor suppressor gene in human lung cancer. Gene silencing by promoter methylation has been observed frequently in adenocarcinoma of the lung, liver, and pancreas. Here, we demonstrate that TSLC1 expression is also absent or markedly reduced in 3 of 4 prostate cancer cell lines. Promoter sequences of TSLC1 were heavily methylated in PPC-1 cells that lacked TSLC1 expression, supporting the idea that promoter methylation is strongly correlated with complete loss of gene expression. Promoter sequences of TSLC1 were also methylated significantly in 7 of 22 (32%) primary prostate cancers. Hypermethylation of the promoter occurred not only in advanced tumors, but also in relatively early-stage tumors. Restoration of TSLC1 expression substantially suppressed tumor formation of PPC-1 cells in nude mice. These findings indicate that alteration of TSLC1 is involved in prostate cancer.

Original languageEnglish (US)
Pages (from-to)605-609
Number of pages5
JournalJapanese Journal of Cancer Research
Volume93
Issue number6
DOIs
StatePublished - Jan 1 2002

Keywords

  • Promoter methylation
  • Prostate cancer
  • Suppresson of tumorigenicity
  • TSLC1
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Fukuhara, H., Kuramochi, M., Fukami, T., Kasahara, K., Furuhata, M., Nobukuni, T., Maruyama, T., Isogai, K., Sekiya, T., Shuin, T., Kitamura, T., Reeves, R. H., & Murakami, Y. (2002). Promoter methylation of TSLC1 and tumor suppression by its gene product in human prostate cancer. Japanese Journal of Cancer Research, 93(6), 605-609. https://doi.org/10.1111/j.1349-7006.2002.tb01297.x