Promoter hypermethylation of the gene encoding heat shock protein B1 in oral squamous carcinoma cells

Hsueh Chun Wang, Wei Fan Chiang, Hsin Hsiu Huang, Shau Ku Huang, Hung Che Chiang

Research output: Contribution to journalArticle

Abstract

Objective: The mechanism responsible for the regulation of HSPB1 expression in oral squamous cell carcinoma was explored in this study. Study Design: The expression and the methylation status of HSPB1 in oral squamous carcinoma cells were examined using real-time reverse transcription-PCR, methylation-specific PCR and pyrosequencing. Results: HSPB1 expression was weakly expressed in oral squamous carcinoma cell lines (N = 4) as compared to that of normal human oral keratinocytes. The lower expressed HSPB1 was associated with promoter hypermethylation of the HSPB1 gene, and the expression of HSPB1 could be induced by treating the cells with a DNA methyltransferase inhibitor, RG108. Promoter hypermethylation of the HSPB1 gene was also noted in primary oral squamous carcinomas, concomitant with reduced levels of HSPB1 gene expression, as compared to those of the paired neighboring normal tissues. Conclusion: Aberrant promoter hypermethylation of the HSPB1 gene may explain the reduced expression of HSPB1 noted in oral cancer cells.

Original languageEnglish (US)
Pages (from-to)376-384
Number of pages9
JournalOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Volume115
Issue number3
DOIs
StatePublished - Mar 1 2013

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Pathology and Forensic Medicine
  • Dentistry (miscellaneous)
  • Radiology Nuclear Medicine and imaging

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