Promoter hypermethylation in Indian primary oral squamous cell carcinoma

Jatinder Kaur, Semra Demokan, Satyendra Chandra Tripathi, Muzafar Ahmad MacHa, Shahnaz Begum, Joseph A. Califano, Ranju Ralhan

Research output: Contribution to journalArticle

Abstract

We evaluated promoter hypermethylation of a panel of tumor suppressor genes as a means to detect epigenetic alterations in oral squamous cell carcinomas (OSCC) of Indian-origin and compare with North-American head and neck squamous cell carcinomas (HNSCC). Quantitative-methylation-specific PCR was used to investigate the promoter methylation status of DCC, EDNRB, p16INK4a and KIF1A in 92 OSCC, and compared to 48 paired normal tissues and 30 saliva and sera samples from healthy control subjects. Aberrant methylation of at-least one of these genes was detected in 74/92 (80.4%) OSCC; 72.8% at EDNRB, 71.7% at KIF1A, 47.8% at p16INK4a and 58.7% at DCC; and in 5 of 48 (10.4%) normal oral tissues. None of the saliva and sera samples from controls exhibited DNA methylation in these four target genes. Thirty-two of 72 node positive cases harbored p16INK4a and DCC hypermethylation (p = 0.005). Thus, promoter hypermethylation in genes analyzed herein is a common event in Indian OSCC and may represent promising markers for the molecular staging of OSCC patients. We found higher frequency of p16INK4a methylation (47.8%) in this Indian cohort in comparison with a North-American cohort (37.5%). In conclusion, aberrant methylation of EDNRB, KIF1A, DCC and p16INK4a genes is a common event in Indian OSCC, suggesting that epigenetic alterations of these genes warrant validation in larger studies for their potential use as biomarkers.

Original languageEnglish (US)
Pages (from-to)2367-2373
Number of pages7
JournalInternational Journal of Cancer
Volume127
Issue number10
DOIs
StatePublished - Nov 15 2010

Fingerprint

Squamous Cell Carcinoma
Methylation
Saliva
Epigenomics
Genes
DCC Genes
p16 Genes
DNA Methylation
Tumor Suppressor Genes
Serum
Healthy Volunteers
Biomarkers
Polymerase Chain Reaction

Keywords

  • DCC
  • EDNRB
  • hypermethylation
  • KIF1A
  • nodal metastasis
  • OSCC
  • p16

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Kaur, J., Demokan, S., Tripathi, S. C., MacHa, M. A., Begum, S., Califano, J. A., & Ralhan, R. (2010). Promoter hypermethylation in Indian primary oral squamous cell carcinoma. International Journal of Cancer, 127(10), 2367-2373. https://doi.org/10.1002/ijc.25377

Promoter hypermethylation in Indian primary oral squamous cell carcinoma. / Kaur, Jatinder; Demokan, Semra; Tripathi, Satyendra Chandra; MacHa, Muzafar Ahmad; Begum, Shahnaz; Califano, Joseph A.; Ralhan, Ranju.

In: International Journal of Cancer, Vol. 127, No. 10, 15.11.2010, p. 2367-2373.

Research output: Contribution to journalArticle

Kaur, J, Demokan, S, Tripathi, SC, MacHa, MA, Begum, S, Califano, JA & Ralhan, R 2010, 'Promoter hypermethylation in Indian primary oral squamous cell carcinoma', International Journal of Cancer, vol. 127, no. 10, pp. 2367-2373. https://doi.org/10.1002/ijc.25377
Kaur J, Demokan S, Tripathi SC, MacHa MA, Begum S, Califano JA et al. Promoter hypermethylation in Indian primary oral squamous cell carcinoma. International Journal of Cancer. 2010 Nov 15;127(10):2367-2373. https://doi.org/10.1002/ijc.25377
Kaur, Jatinder ; Demokan, Semra ; Tripathi, Satyendra Chandra ; MacHa, Muzafar Ahmad ; Begum, Shahnaz ; Califano, Joseph A. ; Ralhan, Ranju. / Promoter hypermethylation in Indian primary oral squamous cell carcinoma. In: International Journal of Cancer. 2010 ; Vol. 127, No. 10. pp. 2367-2373.
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abstract = "We evaluated promoter hypermethylation of a panel of tumor suppressor genes as a means to detect epigenetic alterations in oral squamous cell carcinomas (OSCC) of Indian-origin and compare with North-American head and neck squamous cell carcinomas (HNSCC). Quantitative-methylation-specific PCR was used to investigate the promoter methylation status of DCC, EDNRB, p16INK4a and KIF1A in 92 OSCC, and compared to 48 paired normal tissues and 30 saliva and sera samples from healthy control subjects. Aberrant methylation of at-least one of these genes was detected in 74/92 (80.4{\%}) OSCC; 72.8{\%} at EDNRB, 71.7{\%} at KIF1A, 47.8{\%} at p16INK4a and 58.7{\%} at DCC; and in 5 of 48 (10.4{\%}) normal oral tissues. None of the saliva and sera samples from controls exhibited DNA methylation in these four target genes. Thirty-two of 72 node positive cases harbored p16INK4a and DCC hypermethylation (p = 0.005). Thus, promoter hypermethylation in genes analyzed herein is a common event in Indian OSCC and may represent promising markers for the molecular staging of OSCC patients. We found higher frequency of p16INK4a methylation (47.8{\%}) in this Indian cohort in comparison with a North-American cohort (37.5{\%}). In conclusion, aberrant methylation of EDNRB, KIF1A, DCC and p16INK4a genes is a common event in Indian OSCC, suggesting that epigenetic alterations of these genes warrant validation in larger studies for their potential use as biomarkers.",
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