Prolyl 4-hydroxylase α-related proteins in Drosophila melanogaster: tissue-specific embryonic expression of the 99F8-9 cluster

Elliott W. Abrams, Deborah J Andrew

Research output: Contribution to journalArticle

Abstract

The extracellular matrix (ECM) is proposed to play critical roles in organ morphogenesis through the stabilization and/or sequestration of signaling factors and adhesion molecules, and by maintaining organ integrity. As a first step toward understanding molecules involved in ECM modification and maturation, we have examined the embryonic expression profiles of ten prolyl 4-hydroxylase α subunit (PH4α)-related genes. Prolyl 4-hydroxylases (PH4) catalyze the formation of 4-hydroxyproline in collagens, the major components of the ECM, and are implicated in the hydroxylation of proline in several other secreted proteins. To date, two α subunit proteins have been described in both humans (PHαI and PHαII) and worms (PHY-1/DPY-18 and PHY-2), whereas only a single Drosophila α subunit has been identified. The ten PH4α-related genes described in this study are clustered in a 183-kb region near the tip of chromosome arm 3R and include the previously described Drosophila α subunit gene. Six of the ten PH4α genes in the cluster have tissue-specific embryonic expression. PH4αSG1 and PH4αSG2 are expressed in the salivary gland, PH4αMP is expressed in mouth-part precursors, PH4αPV is expressed in the proventriculus, and CG9698-E is expressed in the epidermis. PH4αEFB is expressed more broadly, with expression in the anterior and posterior midgut primordia, the fat body, the hemocytes and the epidermis. The expression profiles of these PH4α-related genes suggest that tissue-specific ECM modifications may be critical to organ formation and/or function.

Original languageEnglish (US)
Pages (from-to)165-171
Number of pages7
JournalMechanisms of Development
Volume112
Issue number1-2
DOIs
StatePublished - 2002

Fingerprint

Prolyl Hydroxylases
Drosophila Proteins
Extracellular Matrix
Epidermis
Genes
Drosophila
Peptide PHI
Proventriculus
Hemocytes
Fat Body
Hydroxyproline
Protein Subunits
Hydroxylation
Multigene Family
Salivary Glands
Morphogenesis
Proline
Mouth
Collagen
Chromosomes

Keywords

  • Collagen
  • Drosophila
  • Endoplasmic reticulum
  • Epidermis
  • Extracellular matrix
  • Fat body
  • Gene complex
  • Hemocytes
  • Midgut
  • Prolyl 4-hydroxylase
  • Proventriculus
  • Salivary gland
  • Tissue-specific

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Prolyl 4-hydroxylase α-related proteins in Drosophila melanogaster : tissue-specific embryonic expression of the 99F8-9 cluster. / Abrams, Elliott W.; Andrew, Deborah J.

In: Mechanisms of Development, Vol. 112, No. 1-2, 2002, p. 165-171.

Research output: Contribution to journalArticle

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