Proline oxidase in cultured mammalian cells

S. J. Downing, J. M. Phang, E. M. Kowaloff, David Valle, R. J. Smith

Research output: Contribution to journalArticle

Abstract

A cultured cell line was chosen with proline oxidase activity, to study the regulation and physiologic role of the enzyme in mammalian tissues. Among the cell lines tested, only LLC-RK 1 cells, derived from rabbit kidney, had significant proline oxidase activity; the Km for proline of the enzyme from these cells was similar to that for the liver enzyme. LLC cells, proline oxidase positive, were able to convert proline to CO 2. In contrast, CHL cells, proline oxidase negative, did not have this capability. The presence of proline oxidase in LLC cells and the absence of the enzyme in fibroblasts suggest that proline oxidase may serve as a marker enzyme for distinguishing parenchymal kidney cells from fibroblasts in culture. Cells transformed by SV40 virus and cells transformed by methylcholanthrene had activities higher than the parent cell line, but this effect of transformation could not be generalized to all transformed cells. Finally, L-hydroxy proline at a 100-fold greater concentration than substrate L-proline failed to decrease proline oxidation. This finding suggests distinct degradative enzymes for these 2 amino acids.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalJournal of Cellular Physiology
Volume91
Issue number3
StatePublished - 1977
Externally publishedYes

Fingerprint

Proline Oxidase
Cultured Cells
Cells
Proline
Enzymes
Fibroblasts
Hydroxyproline
Cell Line
Methylcholanthrene
Carbon Monoxide
Viruses
Cell culture
Liver
Kidney
Simian virus 40
Tissue
Amino Acids
Oxidation
Substrates

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Downing, S. J., Phang, J. M., Kowaloff, E. M., Valle, D., & Smith, R. J. (1977). Proline oxidase in cultured mammalian cells. Journal of Cellular Physiology, 91(3), 369-376.

Proline oxidase in cultured mammalian cells. / Downing, S. J.; Phang, J. M.; Kowaloff, E. M.; Valle, David; Smith, R. J.

In: Journal of Cellular Physiology, Vol. 91, No. 3, 1977, p. 369-376.

Research output: Contribution to journalArticle

Downing, SJ, Phang, JM, Kowaloff, EM, Valle, D & Smith, RJ 1977, 'Proline oxidase in cultured mammalian cells', Journal of Cellular Physiology, vol. 91, no. 3, pp. 369-376.
Downing SJ, Phang JM, Kowaloff EM, Valle D, Smith RJ. Proline oxidase in cultured mammalian cells. Journal of Cellular Physiology. 1977;91(3):369-376.
Downing, S. J. ; Phang, J. M. ; Kowaloff, E. M. ; Valle, David ; Smith, R. J. / Proline oxidase in cultured mammalian cells. In: Journal of Cellular Physiology. 1977 ; Vol. 91, No. 3. pp. 369-376.
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