Proliferative characteristics of human haemopoietic cells in culture

P. E. Crossen, M. P. Bodger, J. M. Trent

Research output: Contribution to journalArticle

Abstract

The proliferative characteristics of haemopoietic cells, either stimulated by PHA or cultured in the mixed colony assay, the CFU-GM assay or in the leukaemic blast cell assay, was studied using BrdU incorporation and sister chromatid differential staining. There were considerable differences between the various cell types in their proliferative characteristics. Cells cultured in the mixed colony assay showed the greatest proliferative activity with 18% of cells completing two cell cycles in 18 h. PHA stimulated lymphocytes and cells cultured in the leukaemic blast cell assay proliferated at a slower rate with 48 and 45% of cells, respectively, completing three cell cycles in 48 h. Bone marrow cells cultured in the CFU-GM assay showed the slowest proliferative activity with only 1% of third division mataphases at 48 h.

Original languageEnglish (US)
Pages (from-to)307-311
Number of pages5
JournalCytobios
Volume43
Issue number174
StatePublished - 1985
Externally publishedYes

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cell culture
Cell Culture Techniques
assay
assays
Granulocyte-Macrophage Progenitor Cells
cells
cultured cells
Cultured Cells
cell cycle
Cell Cycle
differential staining
Chromatids
chromatids
bone marrow cells
Bromodeoxyuridine
Bone Marrow Cells
bone
lymphocytes
Lymphocytes
Staining and Labeling

ASJC Scopus subject areas

  • Aquatic Science
  • Cell Biology

Cite this

Crossen, P. E., Bodger, M. P., & Trent, J. M. (1985). Proliferative characteristics of human haemopoietic cells in culture. Cytobios, 43(174), 307-311.

Proliferative characteristics of human haemopoietic cells in culture. / Crossen, P. E.; Bodger, M. P.; Trent, J. M.

In: Cytobios, Vol. 43, No. 174, 1985, p. 307-311.

Research output: Contribution to journalArticle

Crossen, PE, Bodger, MP & Trent, JM 1985, 'Proliferative characteristics of human haemopoietic cells in culture', Cytobios, vol. 43, no. 174, pp. 307-311.
Crossen PE, Bodger MP, Trent JM. Proliferative characteristics of human haemopoietic cells in culture. Cytobios. 1985;43(174):307-311.
Crossen, P. E. ; Bodger, M. P. ; Trent, J. M. / Proliferative characteristics of human haemopoietic cells in culture. In: Cytobios. 1985 ; Vol. 43, No. 174. pp. 307-311.
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