Proliferative characteristics and sister chromatid exchange (SCE) of colony-forming cells (CFU-S) in acute myelogenous leukemia

Peter E. Crossen; Rebecca D. Broderick; Brian G.M. Durie; Jeffrey M. Trent

doi:10.1016/0165-4608(85)90192-X

Proliferative characteristics and sister chromatid exchange (SCE) of colony-forming cells (CFU-S) in acute myelogenous leukemia

Peter E. Crossen, Rebecca D. Broderick, Brian G.M. Durie, Jeffrey M. Trent

Research output: Contribution to journal › Article › peer-review

Abstract

Sister chromatid exchange (SCE) and cell cycle progression of colony-forming cells (CFU-S) from patients with acute myelogenous leukemia were studied using bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. The mean SCE rate of CFU-S was 7.99, which is similar to that reported for human peripheral blood lymphocytes. Cultures treated with BrdU at culture initiation and then harvested 72-120 hr later indicated a cell cycle time (T_C) of ∼60 hr. However, cells allowed to proliferate for 72-120 hr prior to the addition of BrdU showed T_C values of 12-36 hr. Analysis of serial cultures from one patient revealed a heterogeneous population with a mean T_C of 19.5 hr. These cell cycle times are considerably shorter than previously published reports and suggest that leukemic cells have the potential to divide rapidly.

Original language	English (US)
Pages (from-to)	267-273
Number of pages	7
Journal	Cancer Genetics and Cytogenetics
Volume	14
Issue number	3-4
DOIs	https://doi.org/10.1016/0165-4608(85)90192-X
State	Published - Jan 15 1985
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1016/0165-4608(85)90192-X

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@article{84c2872fd7754511a0e6452f34066ae1,

title = "Proliferative characteristics and sister chromatid exchange (SCE) of colony-forming cells (CFU-S) in acute myelogenous leukemia",

abstract = "Sister chromatid exchange (SCE) and cell cycle progression of colony-forming cells (CFU-S) from patients with acute myelogenous leukemia were studied using bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. The mean SCE rate of CFU-S was 7.99, which is similar to that reported for human peripheral blood lymphocytes. Cultures treated with BrdU at culture initiation and then harvested 72-120 hr later indicated a cell cycle time (TC) of ∼60 hr. However, cells allowed to proliferate for 72-120 hr prior to the addition of BrdU showed TC values of 12-36 hr. Analysis of serial cultures from one patient revealed a heterogeneous population with a mean TC of 19.5 hr. These cell cycle times are considerably shorter than previously published reports and suggest that leukemic cells have the potential to divide rapidly.",

author = "Crossen, {Peter E.} and Broderick, {Rebecca D.} and Durie, {Brian G.M.} and Trent, {Jeffrey M.}",

note = "Funding Information: J. M. T. is supported in part by PHHS Grants CA-29476, CA-17094, and CA-23074. P. E. C. is supported by the Cancer Society of New Zealand and American Cancer Society Grant IN-11OG. J. M. T. is a Scholar of the Leukemia Society of America. We gratefully acknowledge the technical assistance of L. Norris-Kloos and M. McFadden and the secretarial assistance of B. J. Salterberg.",

year = "1985",

month = jan,

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doi = "10.1016/0165-4608(85)90192-X",

language = "English (US)",

volume = "14",

pages = "267--273",

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AU - Crossen, Peter E.

AU - Broderick, Rebecca D.

AU - Durie, Brian G.M.

AU - Trent, Jeffrey M.

N1 - Funding Information: J. M. T. is supported in part by PHHS Grants CA-29476, CA-17094, and CA-23074. P. E. C. is supported by the Cancer Society of New Zealand and American Cancer Society Grant IN-11OG. J. M. T. is a Scholar of the Leukemia Society of America. We gratefully acknowledge the technical assistance of L. Norris-Kloos and M. McFadden and the secretarial assistance of B. J. Salterberg.

PY - 1985/1/15

Y1 - 1985/1/15

N2 - Sister chromatid exchange (SCE) and cell cycle progression of colony-forming cells (CFU-S) from patients with acute myelogenous leukemia were studied using bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. The mean SCE rate of CFU-S was 7.99, which is similar to that reported for human peripheral blood lymphocytes. Cultures treated with BrdU at culture initiation and then harvested 72-120 hr later indicated a cell cycle time (TC) of ∼60 hr. However, cells allowed to proliferate for 72-120 hr prior to the addition of BrdU showed TC values of 12-36 hr. Analysis of serial cultures from one patient revealed a heterogeneous population with a mean TC of 19.5 hr. These cell cycle times are considerably shorter than previously published reports and suggest that leukemic cells have the potential to divide rapidly.

AB - Sister chromatid exchange (SCE) and cell cycle progression of colony-forming cells (CFU-S) from patients with acute myelogenous leukemia were studied using bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. The mean SCE rate of CFU-S was 7.99, which is similar to that reported for human peripheral blood lymphocytes. Cultures treated with BrdU at culture initiation and then harvested 72-120 hr later indicated a cell cycle time (TC) of ∼60 hr. However, cells allowed to proliferate for 72-120 hr prior to the addition of BrdU showed TC values of 12-36 hr. Analysis of serial cultures from one patient revealed a heterogeneous population with a mean TC of 19.5 hr. These cell cycle times are considerably shorter than previously published reports and suggest that leukemic cells have the potential to divide rapidly.

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Proliferative characteristics and sister chromatid exchange (SCE) of colony-forming cells (CFU-S) in acute myelogenous leukemia

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