Proliferating cellular nuclear antigen expression as a marker of perivascular macrophages in simian immunodeficiency virus encephalitis

Kenneth C. Williams, Annette Schwartz, Sarah Corey, Marlene Orandle, William Kennedy, Brendon Thompson, Xavier Alvarez, Charlie Brown, Suzanne Gartner, Andrew Lackner

Research output: Contribution to journalArticle

Abstract

Brain perivascular macrophages are a major target of simian immunodeficiency virus (SIV) infection in rhesus macaques and HIV infection in humans. Perivascular macrophages are distinct from parenchymal microglia in their location, morphology, expression of myeloid markers, and turnover in the CNS. In contrast to parenchymal microglia, perivascular macrophages are continuously repopulated by blood monocytes, which undergo maturation to macrophages on entering the central nervous system (CNS). We studied differences in monocyte/macrophages in vivo that might account for preferential infection of perivascular macrophages by SIV. In situ hybridization for SIV and proliferating cellular nuclear antigen (PCNA) immunohistochemistry demonstrated that SIV-infected and PCNA-positive cells were predominantly found in perivascular cuffs of viremic animals and in histopathological lesions that characterize SIV encephalitis (SIVE) in animals with AIDS. Multilabel techniques including double-label immunohistochemistry and combined in situ hybridization and immunofluorescence confocal microscopy revealed numerous infected perivascular macrophages that were PCNA-positive. Outside the CNS, SIV-infected, PCNA-expressing macrophage subpopulations were found in the small intestine and lung of animals with AIDS. While PCNA is used as a marker of cell proliferation it is also strongly expressed in non-dividing cells undergoing DNA synthesis and repair. Therefore, more specific markers for cell proliferation including Ki-67, topoisomerase IIα, and bromodeoxyuridine (BrdU) incorporation were used which indicated that PCNA-positive cells within SIVE lesions were not proliferating. These observations are consistent with perivascular macrophages as terminally differentiated, non-dividing cells and underscores biological differences that could potentially define mechanisms of preferential, productive infection of perivascular macrophages in the rhesus macaque model of neuroAIDS. These studies suggest that within CNS and non-CNS tissues there exist subpopulations of macrophages that are SIV-infected and express PCNA.

Original languageEnglish (US)
Pages (from-to)575-585
Number of pages11
JournalAmerican Journal of Pathology
Volume161
Issue number2
StatePublished - 2002

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Simian Immunodeficiency Virus
Nuclear Antigens
Encephalitis
Macrophages
Central Nervous System
Microglia
Macaca mulatta
In Situ Hybridization
Monocytes
Acquired Immunodeficiency Syndrome
Immunohistochemistry
Cell Proliferation
Nerve Tissue
Type II DNA Topoisomerase
Virus Diseases
Bromodeoxyuridine
Infection
Fluorescence Microscopy
Confocal Microscopy
DNA Repair

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Williams, K. C., Schwartz, A., Corey, S., Orandle, M., Kennedy, W., Thompson, B., ... Lackner, A. (2002). Proliferating cellular nuclear antigen expression as a marker of perivascular macrophages in simian immunodeficiency virus encephalitis. American Journal of Pathology, 161(2), 575-585.

Proliferating cellular nuclear antigen expression as a marker of perivascular macrophages in simian immunodeficiency virus encephalitis. / Williams, Kenneth C.; Schwartz, Annette; Corey, Sarah; Orandle, Marlene; Kennedy, William; Thompson, Brendon; Alvarez, Xavier; Brown, Charlie; Gartner, Suzanne; Lackner, Andrew.

In: American Journal of Pathology, Vol. 161, No. 2, 2002, p. 575-585.

Research output: Contribution to journalArticle

Williams, KC, Schwartz, A, Corey, S, Orandle, M, Kennedy, W, Thompson, B, Alvarez, X, Brown, C, Gartner, S & Lackner, A 2002, 'Proliferating cellular nuclear antigen expression as a marker of perivascular macrophages in simian immunodeficiency virus encephalitis', American Journal of Pathology, vol. 161, no. 2, pp. 575-585.
Williams, Kenneth C. ; Schwartz, Annette ; Corey, Sarah ; Orandle, Marlene ; Kennedy, William ; Thompson, Brendon ; Alvarez, Xavier ; Brown, Charlie ; Gartner, Suzanne ; Lackner, Andrew. / Proliferating cellular nuclear antigen expression as a marker of perivascular macrophages in simian immunodeficiency virus encephalitis. In: American Journal of Pathology. 2002 ; Vol. 161, No. 2. pp. 575-585.
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