Programmed cell death and the morphogenesis of the hindbrain roof plate in the chick embryo

Aaron Lawson, Gary C. Schoenwolf, Marjorie A. England, Frederick K. Addai, Rexford S Ahima

Research output: Contribution to journalArticle

Abstract

The spatial and temporal distribution of apoptosis in the dorsal midline of the developing chick hind-brain was examined in relation to the development of the neuroepithelium and neural crest using scanning and transmission electron microscopy, immunocytochemistry and in situ hybridization. The pattern of TUNEL labeling and Slug expression in the dorsal midline at stages 10 and 11 differed from that at stages 12-15. At stages 10 and 11, TUNEL labeling and Slug expression were observed in the dorsal part of location II of rhombomere 1/2 (i.e., between the surface ectoderm and the neuroepithelium), but from stage 12 onward, they were observed in both the dorsal and ventral parts of location II. The implication is that whereas apoptosis may be restricted to a subpopulation of the early migrating neural crest at stages 10 and 11, it presumably occurs in subpopulations of both neural crest and neuroepithelial cells from stage 12 onward. Furthermore, as judged by the pattern of TUNEL labeling and Slug expression in r3 and r5, apoptosis in these two rhombomeres likely occurs in subpopulations of both neural crest and neuroepithelial cells. The eminence present in location I of r1/r2 between stages 10 and 12 consisted of both neural crest and neuroepithelial cells. These cells gradually underwent apoptosis until stage 12, when the eminence disappeared in most embryos. The formation of the inner (neuroepithelial) aspect of the hindbrain roof plate involved both cell migration from adjacent neuroepithelium and an alteration in the shapes of the cells, such that cells with flattened surfaces eventually lined the roof plate. During these processes, some of the neuroepithelial cells underwent apoptosis (i.e., in location IV). The results of this study thus demonstrate that subpopulations of both neuroepithelial and neural crest cells may be involved in programmed cell death in the hindbrain. Additionally, apoptosis in the hindbrain contributes significantly to morphogenetic thinning during roof plate formation.

Original languageEnglish (US)
Pages (from-to)509-519
Number of pages11
JournalAnatomy and Embryology
Volume200
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Rhombencephalon
Neural Crest
Chick Embryo
Morphogenesis
Neuroepithelial Cells
Cell Death
Apoptosis
Gastropoda
In Situ Nick-End Labeling
Scanning Transmission Electron Microscopy
Ectoderm
Cell Shape
Cell Movement
In Situ Hybridization
Embryonic Structures
Immunohistochemistry

Keywords

  • Apoptosis
  • Neural crest cells
  • Neuroepithelium
  • Neurulation

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology
  • Embryology

Cite this

Programmed cell death and the morphogenesis of the hindbrain roof plate in the chick embryo. / Lawson, Aaron; Schoenwolf, Gary C.; England, Marjorie A.; Addai, Frederick K.; Ahima, Rexford S.

In: Anatomy and Embryology, Vol. 200, No. 5, 1999, p. 509-519.

Research output: Contribution to journalArticle

Lawson, Aaron ; Schoenwolf, Gary C. ; England, Marjorie A. ; Addai, Frederick K. ; Ahima, Rexford S. / Programmed cell death and the morphogenesis of the hindbrain roof plate in the chick embryo. In: Anatomy and Embryology. 1999 ; Vol. 200, No. 5. pp. 509-519.
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AB - The spatial and temporal distribution of apoptosis in the dorsal midline of the developing chick hind-brain was examined in relation to the development of the neuroepithelium and neural crest using scanning and transmission electron microscopy, immunocytochemistry and in situ hybridization. The pattern of TUNEL labeling and Slug expression in the dorsal midline at stages 10 and 11 differed from that at stages 12-15. At stages 10 and 11, TUNEL labeling and Slug expression were observed in the dorsal part of location II of rhombomere 1/2 (i.e., between the surface ectoderm and the neuroepithelium), but from stage 12 onward, they were observed in both the dorsal and ventral parts of location II. The implication is that whereas apoptosis may be restricted to a subpopulation of the early migrating neural crest at stages 10 and 11, it presumably occurs in subpopulations of both neural crest and neuroepithelial cells from stage 12 onward. Furthermore, as judged by the pattern of TUNEL labeling and Slug expression in r3 and r5, apoptosis in these two rhombomeres likely occurs in subpopulations of both neural crest and neuroepithelial cells. The eminence present in location I of r1/r2 between stages 10 and 12 consisted of both neural crest and neuroepithelial cells. These cells gradually underwent apoptosis until stage 12, when the eminence disappeared in most embryos. The formation of the inner (neuroepithelial) aspect of the hindbrain roof plate involved both cell migration from adjacent neuroepithelium and an alteration in the shapes of the cells, such that cells with flattened surfaces eventually lined the roof plate. During these processes, some of the neuroepithelial cells underwent apoptosis (i.e., in location IV). The results of this study thus demonstrate that subpopulations of both neuroepithelial and neural crest cells may be involved in programmed cell death in the hindbrain. Additionally, apoptosis in the hindbrain contributes significantly to morphogenetic thinning during roof plate formation.

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