Prognostic value of morphometric features and the callender classification in uveal melanomas

K. Coleman, J. P A Baak, P. J. Van Diest, J. Mullaney

Research output: Contribution to journalArticle

Abstract

Purpose: Cytomorphometric analysis of uveal melanoma has focused on nucleolar characteristics, although nuclear measurements are easier and more reproducible. In this study, we examine a restricted set of nuclear and nucleolar cytomorphometric variables in the search for the most quantifiable and reproducible prognostic parameter among these and discover a means of assessing the malignancy of tumors within the different Callender subgroups. Methods: Uveal melanomas from 94 patients with a minimum follow-up of 5 years underwent nuclear and nucleolar morphometric analysis using a digitized interactive video overlay system. Statistical analysis included univariate and multivariate survival analysis on morphometric variables and classic prognostic parameters (such as Callender cell type, size, etc.). Results: Univariate analysis of cytomorphometric parameters showed the standard deviation of nuclear area (NASD) to be the most significant variable (P <0.0001; Mantel-Cox, 15.2). This was followed by the difference between nuclear and nucleolar areas (P <0.002; Mantel-Cox, 9.5), the standard deviation of nucleolar area (SDNA) (P <0.01; Mantel-Cox, 6.4), and the standard deviation of the shortest nuclear axis (P <0.02; Mantel-Cox, 6.4). There was a trend for significance with the mean of the ten largest nucleoli (P <0.07). Multivariate analysis of clinical and cytomorphometric variables showed improvement of prognostic prediction with a combination of the NASD, largest tumor dimension, and glaucoma (P <0.0001; Mantel-Cox, 26.3). Callender cell type was strongly correlated with the NASD (P <0.0002), the mean axis ratio of the nuclear area (P <0.0007), and the mean of the ten largest nucleoli (P <0.0007). Conclusion: Cytomorphometric analysis of uveal melanoma should include nuclear area and may identify tumors of differing malignancy within the same Callender class.

Original languageEnglish (US)
Pages (from-to)1634-1641
Number of pages8
JournalOphthalmology
Volume103
Issue number10
StatePublished - 1996
Externally publishedYes

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Neoplasms
Multivariate Analysis
Survival Analysis
Cell Size
Glaucoma
Uveal melanoma

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Coleman, K., Baak, J. P. A., Van Diest, P. J., & Mullaney, J. (1996). Prognostic value of morphometric features and the callender classification in uveal melanomas. Ophthalmology, 103(10), 1634-1641.

Prognostic value of morphometric features and the callender classification in uveal melanomas. / Coleman, K.; Baak, J. P A; Van Diest, P. J.; Mullaney, J.

In: Ophthalmology, Vol. 103, No. 10, 1996, p. 1634-1641.

Research output: Contribution to journalArticle

Coleman, K, Baak, JPA, Van Diest, PJ & Mullaney, J 1996, 'Prognostic value of morphometric features and the callender classification in uveal melanomas', Ophthalmology, vol. 103, no. 10, pp. 1634-1641.
Coleman K, Baak JPA, Van Diest PJ, Mullaney J. Prognostic value of morphometric features and the callender classification in uveal melanomas. Ophthalmology. 1996;103(10):1634-1641.
Coleman, K. ; Baak, J. P A ; Van Diest, P. J. ; Mullaney, J. / Prognostic value of morphometric features and the callender classification in uveal melanomas. In: Ophthalmology. 1996 ; Vol. 103, No. 10. pp. 1634-1641.
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