Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer

Eva Shrestha, James R. White, Shu Han Yu, Ibrahim Kulac, Onur Ertunc, Angelo Michael Demarzo, S Yegnasubramanian, Leslie A. Mangold, Alan Wayne Partin, Karen Sfanos

Research output: Contribution to journalArticle

Abstract

Purpose Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer. Materials and Methods Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina® sequencing and 16S rDNA quantitative polymerase chain reaction. We determined the association between bacterial species and the presence or absence of cancer, cancer grade, and type and degree of prostate inflammation. Results Urine samples revealed diverse bacterial populations. There were no significant differences in α or β diversity and no clear hierarchical clustering of benign or cancer samples. We identified a cluster of pro-inflammatory bacteria previously implicated in urogenital infections in a subset of samples. Many species, including known uropathogens, were significantly and differentially abundant among cancer and benign samples, in low vs higher grade cancers and in relation to prostate inflammation type and degree. Conclusions To our knowledge we report the most comprehensive study to date of the male urinary microbiome and its relationship to prostate cancer. Our results suggest a prevalence of pro-inflammatory bacteria and uropathogens in the urinary tract of men with prostate cancer.

Original languageEnglish (US)
Pages (from-to)161-171
Number of pages11
JournalJournal of Urology
Volume199
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Microbiota
Prostatic Neoplasms
Urine
Biopsy
Prostate
Neoplasms
Ribosomal DNA
Inflammation
Bacteria
Dysbiosis
Bacterial DNA
Urinary Tract
Cluster Analysis
Polymerase Chain Reaction
DNA
Infection
Population

Keywords

  • bacteria
  • inflammation
  • microbiota
  • prostatic neoplasms
  • urinary tract

ASJC Scopus subject areas

  • Urology

Cite this

Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer. / Shrestha, Eva; White, James R.; Yu, Shu Han; Kulac, Ibrahim; Ertunc, Onur; Demarzo, Angelo Michael; Yegnasubramanian, S; Mangold, Leslie A.; Partin, Alan Wayne; Sfanos, Karen.

In: Journal of Urology, Vol. 199, No. 1, 01.01.2018, p. 161-171.

Research output: Contribution to journalArticle

Shrestha, Eva ; White, James R. ; Yu, Shu Han ; Kulac, Ibrahim ; Ertunc, Onur ; Demarzo, Angelo Michael ; Yegnasubramanian, S ; Mangold, Leslie A. ; Partin, Alan Wayne ; Sfanos, Karen. / Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer. In: Journal of Urology. 2018 ; Vol. 199, No. 1. pp. 161-171.
@article{b3bbf0a377a745c89e743ae534c0c8fb,
title = "Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer",
abstract = "Purpose Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer. Materials and Methods Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina{\circledR} sequencing and 16S rDNA quantitative polymerase chain reaction. We determined the association between bacterial species and the presence or absence of cancer, cancer grade, and type and degree of prostate inflammation. Results Urine samples revealed diverse bacterial populations. There were no significant differences in α or β diversity and no clear hierarchical clustering of benign or cancer samples. We identified a cluster of pro-inflammatory bacteria previously implicated in urogenital infections in a subset of samples. Many species, including known uropathogens, were significantly and differentially abundant among cancer and benign samples, in low vs higher grade cancers and in relation to prostate inflammation type and degree. Conclusions To our knowledge we report the most comprehensive study to date of the male urinary microbiome and its relationship to prostate cancer. Our results suggest a prevalence of pro-inflammatory bacteria and uropathogens in the urinary tract of men with prostate cancer.",
keywords = "bacteria, inflammation, microbiota, prostatic neoplasms, urinary tract",
author = "Eva Shrestha and White, {James R.} and Yu, {Shu Han} and Ibrahim Kulac and Onur Ertunc and Demarzo, {Angelo Michael} and S Yegnasubramanian and Mangold, {Leslie A.} and Partin, {Alan Wayne} and Karen Sfanos",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.juro.2017.08.001",
language = "English (US)",
volume = "199",
pages = "161--171",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer

AU - Shrestha, Eva

AU - White, James R.

AU - Yu, Shu Han

AU - Kulac, Ibrahim

AU - Ertunc, Onur

AU - Demarzo, Angelo Michael

AU - Yegnasubramanian, S

AU - Mangold, Leslie A.

AU - Partin, Alan Wayne

AU - Sfanos, Karen

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Purpose Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer. Materials and Methods Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina® sequencing and 16S rDNA quantitative polymerase chain reaction. We determined the association between bacterial species and the presence or absence of cancer, cancer grade, and type and degree of prostate inflammation. Results Urine samples revealed diverse bacterial populations. There were no significant differences in α or β diversity and no clear hierarchical clustering of benign or cancer samples. We identified a cluster of pro-inflammatory bacteria previously implicated in urogenital infections in a subset of samples. Many species, including known uropathogens, were significantly and differentially abundant among cancer and benign samples, in low vs higher grade cancers and in relation to prostate inflammation type and degree. Conclusions To our knowledge we report the most comprehensive study to date of the male urinary microbiome and its relationship to prostate cancer. Our results suggest a prevalence of pro-inflammatory bacteria and uropathogens in the urinary tract of men with prostate cancer.

AB - Purpose Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer. Materials and Methods Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina® sequencing and 16S rDNA quantitative polymerase chain reaction. We determined the association between bacterial species and the presence or absence of cancer, cancer grade, and type and degree of prostate inflammation. Results Urine samples revealed diverse bacterial populations. There were no significant differences in α or β diversity and no clear hierarchical clustering of benign or cancer samples. We identified a cluster of pro-inflammatory bacteria previously implicated in urogenital infections in a subset of samples. Many species, including known uropathogens, were significantly and differentially abundant among cancer and benign samples, in low vs higher grade cancers and in relation to prostate inflammation type and degree. Conclusions To our knowledge we report the most comprehensive study to date of the male urinary microbiome and its relationship to prostate cancer. Our results suggest a prevalence of pro-inflammatory bacteria and uropathogens in the urinary tract of men with prostate cancer.

KW - bacteria

KW - inflammation

KW - microbiota

KW - prostatic neoplasms

KW - urinary tract

UR - http://www.scopus.com/inward/record.url?scp=85034098046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034098046&partnerID=8YFLogxK

U2 - 10.1016/j.juro.2017.08.001

DO - 10.1016/j.juro.2017.08.001

M3 - Article

C2 - 28797714

AN - SCOPUS:85034098046

VL - 199

SP - 161

EP - 171

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 1

ER -