Profiling the expression pattern of GPI transamidase complex subunits in human cancer

Jatin K. Nagpal; Santanu Dasgupta; Sana Jadallah; Young K. Chae; Edward A. Ratovitski; Antoun Toubaji; George J. Netto; Toby Eagle; Aviram Nissan; David Sidransky; Barry Trink

doi:10.1038/modpathol.2008.76

Profiling the expression pattern of GPI transamidase complex subunits in human cancer

Jatin K. Nagpal, Santanu Dasgupta, Sana Jadallah, Young K. Chae, Edward A. Ratovitski, Antoun Toubaji, George J. Netto, Toby Eagle, Aviram Nissan, David Sidransky, Barry Trink

School of Medicine

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The glycosylphosphatidylinositol transamidase complex (GPIT) consists of five subunits: PIG-U, PIG-T, GPAA1, PIG-S and GPI8, and is important in attaching GPI anchors to target proteins. On the basis of our previous reports incriminating PIG-U as an oncogene in bladder cancer and PIG-T and GPAA1 as oncogenes in breast cancer, we evaluated the expression pattern of the GPIT subunits in 19 different human cancers at both mRNA and protein levels. In general, our results demonstrate a more frequent expression of GPIT subunits in cancers than in normal. Among the 19 anatomic sites compared; breast, ovary and uterus showed consistent evidence of overexpression of specific GPIT subunits. There was also overexpression of PIG-U and GPI8 in lymphoma. In addition, non-small cell lung carcinoma showed significant overexpression of the GPIT subunits as compared to small cell lung carcinoma and normal lung tissue. Also, deregulation of specific GPIT subunits was seen in various other cancers. Forced overexpression of two GPIT subunits; PIG-S and GPI8 alone or in combination induced increased proliferation and invasion of breast cancer cells. Collectively, our study defines a trend involving the deregulated expression and the functional contribution of the GPIT subunits in various cancers with potential implications in diagnosis, prognosis and therapeutic intervention.

Original language	English (US)
Pages (from-to)	979-991
Number of pages	13
Journal	Modern Pathology
Volume	21
Issue number	8
DOIs	https://doi.org/10.1038/modpathol.2008.76
State	Published - Aug 2008

Keywords

Cancer
GPIT complex
Gene expression

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1038/modpathol.2008.76

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@article{a35a22857a9c421d899d2bdb5f2d1c9f,

title = "Profiling the expression pattern of GPI transamidase complex subunits in human cancer",

abstract = "The glycosylphosphatidylinositol transamidase complex (GPIT) consists of five subunits: PIG-U, PIG-T, GPAA1, PIG-S and GPI8, and is important in attaching GPI anchors to target proteins. On the basis of our previous reports incriminating PIG-U as an oncogene in bladder cancer and PIG-T and GPAA1 as oncogenes in breast cancer, we evaluated the expression pattern of the GPIT subunits in 19 different human cancers at both mRNA and protein levels. In general, our results demonstrate a more frequent expression of GPIT subunits in cancers than in normal. Among the 19 anatomic sites compared; breast, ovary and uterus showed consistent evidence of overexpression of specific GPIT subunits. There was also overexpression of PIG-U and GPI8 in lymphoma. In addition, non-small cell lung carcinoma showed significant overexpression of the GPIT subunits as compared to small cell lung carcinoma and normal lung tissue. Also, deregulation of specific GPIT subunits was seen in various other cancers. Forced overexpression of two GPIT subunits; PIG-S and GPI8 alone or in combination induced increased proliferation and invasion of breast cancer cells. Collectively, our study defines a trend involving the deregulated expression and the functional contribution of the GPIT subunits in various cancers with potential implications in diagnosis, prognosis and therapeutic intervention.",

keywords = "Cancer, GPIT complex, Gene expression",

author = "Nagpal, {Jatin K.} and Santanu Dasgupta and Sana Jadallah and Chae, {Young K.} and Ratovitski, {Edward A.} and Antoun Toubaji and Netto, {George J.} and Toby Eagle and Aviram Nissan and David Sidransky and Barry Trink",

note = "Funding Information: This study was supported by National Cancer Institute Lung Cancer Specialized program of Research Excellence Grant (CA 58184-01 to DS), High Throughput Genetic Analysis of Bladder Cancer Grant (CA 077664-08 to DS) and by a grant from the Flight Attendant Medical Research Institute (FAM-RI) to BT. We dedicate this work to the memory of our colleague Toby Eagle.",

year = "2008",

month = aug,

doi = "10.1038/modpathol.2008.76",

language = "English (US)",

volume = "21",

pages = "979--991",

journal = "Modern Pathology",

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T1 - Profiling the expression pattern of GPI transamidase complex subunits in human cancer

AU - Nagpal, Jatin K.

AU - Dasgupta, Santanu

AU - Jadallah, Sana

AU - Chae, Young K.

AU - Ratovitski, Edward A.

AU - Toubaji, Antoun

AU - Netto, George J.

AU - Eagle, Toby

AU - Nissan, Aviram

AU - Sidransky, David

AU - Trink, Barry

N1 - Funding Information: This study was supported by National Cancer Institute Lung Cancer Specialized program of Research Excellence Grant (CA 58184-01 to DS), High Throughput Genetic Analysis of Bladder Cancer Grant (CA 077664-08 to DS) and by a grant from the Flight Attendant Medical Research Institute (FAM-RI) to BT. We dedicate this work to the memory of our colleague Toby Eagle.

PY - 2008/8

Y1 - 2008/8

N2 - The glycosylphosphatidylinositol transamidase complex (GPIT) consists of five subunits: PIG-U, PIG-T, GPAA1, PIG-S and GPI8, and is important in attaching GPI anchors to target proteins. On the basis of our previous reports incriminating PIG-U as an oncogene in bladder cancer and PIG-T and GPAA1 as oncogenes in breast cancer, we evaluated the expression pattern of the GPIT subunits in 19 different human cancers at both mRNA and protein levels. In general, our results demonstrate a more frequent expression of GPIT subunits in cancers than in normal. Among the 19 anatomic sites compared; breast, ovary and uterus showed consistent evidence of overexpression of specific GPIT subunits. There was also overexpression of PIG-U and GPI8 in lymphoma. In addition, non-small cell lung carcinoma showed significant overexpression of the GPIT subunits as compared to small cell lung carcinoma and normal lung tissue. Also, deregulation of specific GPIT subunits was seen in various other cancers. Forced overexpression of two GPIT subunits; PIG-S and GPI8 alone or in combination induced increased proliferation and invasion of breast cancer cells. Collectively, our study defines a trend involving the deregulated expression and the functional contribution of the GPIT subunits in various cancers with potential implications in diagnosis, prognosis and therapeutic intervention.

AB - The glycosylphosphatidylinositol transamidase complex (GPIT) consists of five subunits: PIG-U, PIG-T, GPAA1, PIG-S and GPI8, and is important in attaching GPI anchors to target proteins. On the basis of our previous reports incriminating PIG-U as an oncogene in bladder cancer and PIG-T and GPAA1 as oncogenes in breast cancer, we evaluated the expression pattern of the GPIT subunits in 19 different human cancers at both mRNA and protein levels. In general, our results demonstrate a more frequent expression of GPIT subunits in cancers than in normal. Among the 19 anatomic sites compared; breast, ovary and uterus showed consistent evidence of overexpression of specific GPIT subunits. There was also overexpression of PIG-U and GPI8 in lymphoma. In addition, non-small cell lung carcinoma showed significant overexpression of the GPIT subunits as compared to small cell lung carcinoma and normal lung tissue. Also, deregulation of specific GPIT subunits was seen in various other cancers. Forced overexpression of two GPIT subunits; PIG-S and GPI8 alone or in combination induced increased proliferation and invasion of breast cancer cells. Collectively, our study defines a trend involving the deregulated expression and the functional contribution of the GPIT subunits in various cancers with potential implications in diagnosis, prognosis and therapeutic intervention.

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ER -

Profiling the expression pattern of GPI transamidase complex subunits in human cancer

Abstract

Keywords

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