Profiling of Bacteria by Fast Atom Bombardment Mass Spectrometry

D. N. Heller; R. J. Cotter; Catherine Fenselau; O. M. Uy

doi:10.1021/ac00150a018

Profiling of Bacteria by Fast Atom Bombardment Mass Spectrometry

D. N. Heller, R. J. Cotter, Catherine Fenselau, O. M. Uy

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Positive and negative Ion fast atom bombardment mass spectra have been obtained from lysed bacteria Introduced without extraction lnto the mass spectrometer. Phospholipids and other polar lipids are found to be selectively desorbed to provide molecular lons consistent with those observed In spectra of pure lipid samples. Three Gram-positive and three Gram-negative bacteria were studied. The anion spectra from these six bacteria are presented and are found to reflect qualitatively their distinctive phospholipid content. Fast atom bombardment mass spectra may serve as an aid In differentiating species.

Original language	English (US)
Pages (from-to)	2806-2809
Number of pages	4
Journal	Analytical chemistry
Volume	59
Issue number	23
DOIs	https://doi.org/10.1021/ac00150a018
State	Published - Jan 1 1987

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "Positive and negative Ion fast atom bombardment mass spectra have been obtained from lysed bacteria Introduced without extraction lnto the mass spectrometer. Phospholipids and other polar lipids are found to be selectively desorbed to provide molecular lons consistent with those observed In spectra of pure lipid samples. Three Gram-positive and three Gram-negative bacteria were studied. The anion spectra from these six bacteria are presented and are found to reflect qualitatively their distinctive phospholipid content. Fast atom bombardment mass spectra may serve as an aid In differentiating species.",

author = "Heller, {D. N.} and Cotter, {R. J.} and Catherine Fenselau and Uy, {O. M.}",

year = "1987",

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AB - Positive and negative Ion fast atom bombardment mass spectra have been obtained from lysed bacteria Introduced without extraction lnto the mass spectrometer. Phospholipids and other polar lipids are found to be selectively desorbed to provide molecular lons consistent with those observed In spectra of pure lipid samples. Three Gram-positive and three Gram-negative bacteria were studied. The anion spectra from these six bacteria are presented and are found to reflect qualitatively their distinctive phospholipid content. Fast atom bombardment mass spectra may serve as an aid In differentiating species.

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