Abstract
Heterogeneity of stem cell population hampers detailed understanding of stem cell biology, such as their differentiation propensity toward different lineages. A single cell transcriptome assay can be a new approach for dissecting individual variation. We have developed the single cell qRT-PCR method, and confirmed that this method works well in several gene expression profiles. In single cell level, each human embryonic stem cell, sorted by OCT4::EGFP positive cells, has high expression in OCT4, but a different level of NANOG expression. Our single cell gene expression assay should be useful to interrogate population heterogeneities.
Original language | English (US) |
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Article number | e51408 |
Journal | Journal of Visualized Experiments |
Issue number | 87 |
DOIs | |
State | Published - 2014 |
Keywords
- Amplification
- FACS
- Heterogeneity
- Human embryonic stem cell
- Issue 87
- Molecular biology
- Reverse transcriptase
- Single cell
- qRT-PCR
ASJC Scopus subject areas
- General Neuroscience
- General Chemical Engineering
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology